不同菌源诱导免疫的黄粉虫抗菌肽提取物对26种病原菌的抑菌活性  

Antimicrobial Activity of the Antimicrobial Peptides Extracted from Immunized Tenebrio molitor Induced by Different Microbial Treatments Against 26 Pathogens

作　　者：谢咸升[1,2] 董建臻[1] 李静[1] 李永利[1] 王圆圆[1] 李小凤[1] 张亚南[1] 

机构地区：[1]河北农业大学植物保护学院,河北保定071001 [2]山西省农业科学院小麦研究所,山西临汾041000

出　　处：《安徽农业科学》2011年第9期5290-5293,共4页Journal of Anhui Agricultural Sciences

基　　金：河北省自然科学基金项目"高活性昆虫抗菌肽的筛选及其对植物致病菌抑菌活性研究"(C200700450)

摘　　要：[目的]探索经不同菌源诱导黄粉虫产生的免疫物质——抗菌肽对病原菌是否存在活性差异。[方法]通过饲细菌、针刺饲真菌法诱导饥饿处理过的黄粉虫幼虫产生免疫反应,研磨、冷冻离心法获得抗菌肽提取物,采用抑菌圈法测定其对26种病原菌抑菌活性,利用三等分法划分4种类型评价抑菌活性及抑菌谱。[结果]经不同菌源诱导黄粉虫所产生的抗菌肽抑菌活性及抑菌谱上均存在明显差异。[结论]生防菌源在诱导昆虫体液免疫物质——抗菌肽表达上具有明显优势。[Objective] The study was to explore whether antimicrobial activity of the antimicrobial peptides extracted from immunized Tenebrio molitor vary with different pathogens as inducers.[Method] By inducing hungry T.molitor larvaes to generate immune response via feeding with bacteria and with fungi or actinomycete after pricking,the antimicrobial peptide extract was obtained by grinding and centrifuging the cultures.Its antimicrobial activity against 26 pathogens was measured by bacteriostatic ring,and evaluated by trisection to four types and inhibitory spectrum.[Result] Both the antimicrobial activity and antimicrobial spectrum of the antimicrobial peptide extract varied remarkably among different pathogens as inducers.[Conclusion] Bio-control used strains have obvious advantage in inducing the insect to express body fluid immunity material-antimicrobial peptides.

关 键 词：黄粉虫 诱导免疫 抗菌肽 抑菌活性 抑菌谱 

分 类 号：S433[农业科学—农业昆虫与害虫防治]