原发性肝癌染色体8p、16q遗传变异的研究  

Study on Genetic Alterations in Chromosomes 8p and 16q in Primary Hepatocellular Carcinoma

作　　者：周后龙[1] 巩丽[1] 李艳红 刘小艳[3] 兰淼[3] 张贺龙[1] 张伟[3] 冯英明[1] 

机构地区：[1]第四军医大学唐都医院肿瘤科,陕西西安710038 [2]第四军医大学唐都医院妇产科,陕西西安710038 [3]第四军医大学唐都医院病理科,陕西西安710038

出　　处：《现代生物医学进展》2011年第5期844-849,共6页Progress in Modern Biomedicine

基　　金：国家自然科学基金资助项目:No.30800147No.30672013

摘　　要：目的:分析人肝癌(HCC)组织中染色体8p、16q部分基因及染色体片段的遗传变异及与临床病理关系,初步筛选HCC相关的抑癌基因。方法:应用聚合酶链反应-变性聚丙烯酰胺凝胶-银染法分析45例HCC组织标本中染色体8p和16q的杂合性丢失(LOH)及微卫星不稳定性(MSI)。结果:发生LOH的总频率为68.89%(31/45),其中D16S511位点的发生LOH率最高为53.33%(24/45),其次是D8S261(39.02%,16/41)和D8S499(34.88%,15/43)。MSI出现的总频率为11.11%(5/45),出现在三个微卫星位点(D8S261、D8S499及D16S511)上。结论:染色体16q23、8p22-21.3及8p12区域的LOH发生频率高,其可能存在与HCC发生发展相关的新的抑癌基因,特定位点的遗传变异可能与HBV感染、临床病理恶性程度等预后因素相关。Objective：To investigate the genetical alterations on chromosomes 8p and 16q in primary hepatocellular carcinoma（HCC）,investigate the relationship between the gentical alterations and clinicopathologic features and try to screened some HCC-related tumor suppressor genes.Methods：Loss of heterozygosity（LOH） and microsatellite instability（MSI） on chromosome 8p and 16q in samples from thirty-five patients with HCC were examined by PCR-denaturing PAGE-silver staining.Results：The overall LOH frequency was 68.89%（31/45） at least one locus of 8 loci on the chromosomes.The frequency of Loci were 53.33%（31/45）,39.02%（16/41）and34.88%（15/43）for D16S511,D8S261 and D8S499,respectively.The frequency of MSI was 11.11%（5/45） and the MSI was distributed on the three microsatellite markers（D16S511、D8S261 and D8S499）.Conclusion：There may be a new putative tumor suppressor gene related to the occurrence and development on specific chromosome region 16q23,8p22-21.3 or 8p12 with high-frequent LOH.The genetic alterations on some specific loci were associated with prognosis factors as the positive HBsAg,differentiated degrees of HCC.

关 键 词：遗传变异 杂合性丢失 微卫星不稳定 肝细胞肝癌 染色体 

分 类 号：R735.7[医药卫生—肿瘤]