Kinetic Parameter of Alfa-amylase and Glucoamylase Enzymatic Reaction on the Glucose Yield from Hydrolyzed Processes of Tapioca Solid Waste  

作　　者：Sri Rachmania Juliastuti Dian Yanuarita Purwaningsih 

机构地区：[1]Department of Chemical Engineering, Institute of Technology Sepuluh Nopember, Jl. Arief Rakhman Hakim, Keputih-Sukolilo,Surabaya 60111, Indonesia [2]Post Graduated Student of Chemical Engineering, Institute of Technology Sepuluh Nopember, Jl. Arief Rakhman Hakim,Keputih-Sukolilo, Surabaya 60111, Indonesia

出　　处：《Journal of Chemistry and Chemical Engineering》2011年第3期195-201,共7页化学与化工（英文版）

摘　　要：The increasing of tapioca production nowadays effected the production of waste. The waste of tapioca industries consists of two kinds, which were liquid waste and solid waste. Further more, tapioca solid waste treatment was ineffective. Weather solid waste produced from the extraction process still contains high concentration of starch that can be used to produce high quality product, for example, bio ethanol or other alternative energy sources. Objective of these experimental work was utilizing solid waste of tapioca industries and looking for the exactly composition of n-amylase and gluco-amylase enzymes on the hydrolysis processes of the solid waste of tapioca. The exact composition from both enzymes can be expected to increase the yield of glucose. Variables of cx-amylase enzyme for this research were 0.3% （w/w） and 0.5% （w/w） with liquefaction time were 1 hour and 1.5 hours, and variables of glucoamylase enzyme were 0.3% （w/w） and 0.5% （w/w）. To achieve these goals, the experimental work was held in laboratory scale with batch process. Firstly, tapioca solid waste was pretreated at 90 ~C and added u-amylase enzyme for 1 hour and 1.5 hours （variable of liquefaction time）. Then, substrate was cooled down to 60 ~C added with proposed variables of glucoamylase enzyme, and was analysed 24 hours after added. This experiment showed the best ratio between a-amylase and glucoamylase enzymes 0.5%：0.5% with 1 hour of liquefaction time. The highest glucose reaches 8.468% and yields 0.892 （g glucose/g starch） with starch conversion of 59.94%. KM = 0.0468 g/mL and rmax = 0.311 g/mL·h,

关 键 词：Starch hydrolysis or-amylase GLUCOAMYLASE GLUCOSE tapioca solid waste. 

分 类 号：TQ925.1[轻工技术与工程—发酵工程] S533[农业科学—作物学]