利用T-RFLP技术对温榆河微生物群落结构研究  

Study of micro-ecosystem of the Wenyu River by terminal restriction fragment length polymorphism analysis

作　　者：董萍[1] 孙寓姣[1] 王红旗[1] 陈利顶[2] 张辉[1] 

机构地区：[1]北京师范大学水科学研究院,北京100875 [2]中国科学院生态环境研究中心,北京100085

出　　处：《中国环境科学》2011年第4期631-636,共6页China Environmental Science

基　　金：国家"十一五"重大科技专项子课题(2008ZX07526-002-02);国家自然科学基金资助项目(50708008)

摘　　要：利用T-RFLP技术(综合运用PCR技术、DNA限制性酶切技术和DNA序列自动分析技术)对温榆河不同尺度、不同时期的微生物群落结构进行了研究.从微生物的种类数、物种丰度、优势菌落、群落结构稳定性等方面特征着手,找出其优势群落;利用两种多样性指数(香侬一威勒多样性指数、辛普森多样性指数)探讨各种微生物群落结构的多样性以及同一群落随时间而发生的变化;比较了微生物指标与水质指标COD之间的变化关系.结果表明,温榆河不同尺度、不同时期的微生物具有明显变化,说明季节性变化对水体中微生物群落产生了明显的影响;T-RF67bp、151bp、440bp、481bp、488bp所代表的微生物群落体现其为优势种群;T-RF为135bp、151bp、440bp的微生物群落对于外界反映最敏感,验证其有可能能作为环境变化的指示性生物;微生物指标与COD呈现明显的正相关.The micro-ecology of Wenyu River in different scales and periods was researched by T-RFLP method.We found its dominant communities based on several factors： the number of species,species abundance,dominant colonies,the stability of community structure of the micro-organisms,etc.And then we studied the diversity of every microbial community structures,as well as the variation over different times of the same one community.Finally,we examined the relevance between the microbial indicators and water quality objectives（COD）.Results indicate the number of microbe in Wenyu River had significant changed in different scales and periods,also show that seasonal changes had an apparent impact on microbial communities in water.Microbial communities of T-RF67bp,151bp,440bp,481bp,488bp represent the dominant species in river;T-RF135bp,151bp,440bp are most sensitive to environment,suggesting they may act as indictors of environment changing.The relationship between Microbial indicators and cod presents positive correlation.This study is the foundation to further understand the structures and functions of whole ecology system.

关 键 词：T-RFLP 微生态系统 群落结构 16SRRNA基因 

分 类 号：X172[环境科学与工程—环境科学]