固相萃取-高效液相色谱检测葡萄酒中罗丹明B  

Determination of Rhodamine B in Red Wine by Solid Phase Extraction-High Performance Liquid Chromatography

作　　者：李小燕[1,2] 李梅[1] 陈其锋[1] 韦升艳[1] 罗予[1] 仝海娟[1] 

机构地区：[1]广西民族大学化学与生态工程学院,广西南宁530006 [2]广西林产化学品开发与应用重点实验室,广西南宁530006

出　　处：《食品科学》2011年第8期238-243,共6页Food Science

基　　金：广西自然科学基金项目(2010GXNSFA013047);广西教育厅科研项目(200911LX84)

摘　　要：建立固相萃取富集净化,高效液相色谱紫外检测葡萄酒中禁用色素罗丹明B的方法。葡萄酒样品中的罗丹明B经C18固相萃取,40%乙醇溶液清洗净化,80%的甲醇溶液洗脱后,用高效液相色谱紫外检测器进行测定。结果表明:罗丹明B在1.0～10.0μg/mL范围呈现良好的线性关系,相关系数0.9924;在1.5～3.5μg/mL添加水平时,平均回收率为72.06%,相对标准偏差为2.87%(n=5);定量测出限为0.25μg/mL。表明该方法灵敏度高,重复性良好,操作简便快速,适用于葡萄酒中罗丹明B的检测。A solid phase extraction followed by high performance liquid chromatographic method was developed for the analysis of the prohibited colorant rhodamine B in red wine.The separation of rhodamine B from wines was achieved on a SupelcleanC18 SPE column washed with 40%ethanol aqueous solution（V/V）and eluted with 80%methanol aqueous solution（V/V）prior to analysis on a high performance liquid chromatography equipped with a UV detector.The calibration curve of the method was linear over the range of 1.0 to 10.0μg/mL with a correlation coefficient of 0.9924.The recoveries for rhodamine B standard spiked in the range of 1.5 to 3.5μg/mL were between 69.67%and 75.00%with a relative standard deviation（RSD）of 2.87%（n=5）. The detection limit was 0.25μg/mL.Owing to advantages of good linearity,low limit of detection,high precision and rapidity, this method is suitable for the determination of rhodamine B in red wine.

关 键 词：固相萃取 高效液相色谱 罗丹明B 葡萄酒 

分 类 号：O657.72[理学—分析化学]