诃子抗氧化活性物质提取工艺与抗氧化活性研究  

Extraction Technology and Antioxidant Activities of BioactiveComponents from Terminalia chebula Retz.

作　　者：江慎华[1,2] 吴士云[2,3] 马海乐[2] 黄星奕[2] 王振斌[2] 廖亮[1] 

机构地区：[1]九江学院生命科学学院,九江332000 [2]江苏大学食品与生物工程学院,镇江212013 [3]安徽科技学院食品药品学院,凤阳233100

出　　处：《农业机械学报》2011年第4期120-126,共7页Transactions of the Chinese Society for Agricultural Machinery

基　　金："十一五"国家科技支撑计划资助项目(2006BAD27B06);中国博士后科学基金资助项目(20100481099);江苏大学博士后科研基金资助项目;九江学院高级专业人才科研启动基金资助项目

摘　　要：对诃子抗氧化活性物质提取工艺、抗氧化活性及其初步物质基础进行了研究。结果表明,最佳提取工艺为:乙醇体积分数50%、液料比20、提取温度60℃、提取次数4次、提取时间7 m in;总还原力、FRAP法抗氧化能力和DPPH自由基清除能力的结果均显示诃子粗提物具有很强的抗氧化活性。生物活性追踪结果发现,诃子抗氧化活性物质主要由存在于乙酸乙酯相的弱极性化合物组成,该相质量浓度为25、50μg/mL样品的总还原力OD700值分别为0.197±0.002和0.380±0.006,FRAP法抗氧化能力OD593分别为0.272±0.023和0.631±0.002,DPPH自由基清除率分别为(86.838±0.600)%和(90.318±0.917)%。相关关系表明,诃子抗氧化活性的主要物质基础为总黄酮和总多酚。The extraction technology and antioxidant activities of the bioactive components from Terminalia chebula Retz.were studied.The results showed that the optimal parameters of the extraction technology were that the concentration of ethanol was 50%,the ratio of extraction solution to sample was 20,the extraction temperature was 60℃,the extraction times was 4 and the extraction time was 7 min.The crude extracts from this plant were proved to have strong antioxidant activities through the results of the reducing power,the FRAP assay and the DPPH radicals scavenging percentages.The bioactive antioxidants of Terminalia chebula Retz.were mainly laid in the ethyl acetate fraction based on the bioassay-guided method.The OD values at 700 nm（reducing power）,FRAP values（OD593 nm） and DPPH radicals-scavenging percentages of this fraction with the concentrations of 25 and 50 μg/mL were 0.197±0.002 and 0.380±0.006,0.272±0.023 and 0.631±0.002,（86.838±0.600） % and（90.318±0.917）%,respectively.The correlation showed that the strong antioxidant activity mainly resulted from the high contents of total phenols and total flavonoids.

关 键 词：诃子 抗氧化 提取工艺 生物活性追踪法 

分 类 号：TS201[轻工技术与工程—食品科学]