气相色谱-质谱法同时测定乳酸和7种糖的研究  

Simultaneous Determination of Lactate and Seven Sugars by Gas Chromatography-mass Spectrometry

作　　者：徐正华[1] 李晓宁[1] 易伦朝[1,3] 谭斌斌[1] 梁逸曾[1] 曾茂茂[1] 何君[2] 

机构地区：[1]中南大学化学化工学院中药现代化研究中心,湖南长沙410083 [2]中国医学科学院实验动物研究所,北京100021 [3]中南大学湘雅医院医学实验研究中心,卫生部肿瘤蛋白质组学重点实验室,湖南长沙410008

出　　处：《分析科学学报》2011年第2期142-146,共5页Journal of Analytical Science

基　　金：中国博士后科学基金(No.200902481);抗肿瘤、糖尿病、神经退行性疾病新药临床前药效学评价技术平台的建立项目(No.2009ZX09303)

摘　　要：建立了气相色谱-质谱(GC-MS)法同时测定乳酸和葡萄糖、葡萄糖-6-磷酸、果糖、半乳糖、乳糖、1,5-脱水山梨醇、山梨醇7种糖的方法。选择核糖醇为内标,进样前先进行肟化反应再进行硅烷化反应。采用DB-5熔融石英毛细管柱,升温程序为初始温度70℃,保持4min,以8℃/min的速率升至300℃,保持3min。在优化测定条件下各对照品得到良好分离,且在测定范围内具有良好线性关系(r2>0.9983),平均加标回收率为74.5%～104.2%,日间相对标准偏差(RSD)与日内RSD小于9.2%。该方法简便、灵敏度高,已成功应用于2型糖尿病大鼠和健康大鼠血清中乳酸和糖类物质的定性定量分析。A method was developed for simultaneous determination of lactate,glucose,glucose-6-phosphate,fructose,galactose,lactose,1,5-anhydro-sorbitol and sorbitol.Adonitol was used as an internal standard.The oximation and silylation of samples were carried out before chromatographic analysis.A DB-5 fused silica capillary column was used for chromatographic separation.The column temperature was maintained at 70℃ for 1 min and then programmed from 70 to 300℃ at the rate of 8℃,then hold 3 min at 300℃.All compounds were baseline separated under the conditions,and all the compounds had good linearity（r20.9925） in the concentration range of detection.The added sample recovery was in the range of 74.5%～104.2%,and the inter-day relative standard deviation（RSD） and intra-day RSD were less than 9.2%.The method is simple,sensitive and has been successfully applied to the determination of lactate and sugars in the serum of type 2 diabetic rats and health controls.

关 键 词：气相色谱-质谱 乳酸 糖 血清 2型糖尿病 

分 类 号：O657.63[理学—分析化学]