乳腺癌细胞exosomes对人脐静脉内皮作用的实验研究  

The experimental study of effect of exosomes derived from breast cancer cell line on human umbilical vein endothelial cells

作　　者：谢莹珊[1] 沈宜[1] 隆霜[1] 孙迪[1] 姜蓉[1] 陈黎[1] 

机构地区：[1]重庆医科大学,重庆400016

出　　处：《激光杂志》2011年第2期71-73,共3页Laser Journal

基　　金：重庆市渝中区科委资助项目(2009)

摘　　要：目的:观察人乳腺癌细胞株MDA-MB-231细胞源exosomes对人脐静脉内皮细胞株(HUVECs)增殖、迁移能力及血管样结构形成的影响。方法:超速离心及密度梯度离心法提取exosomes;MTT法检测MDA-MB-231细胞源exosomes对HUVECs增殖的影响;Transwell小室法检测HUVECs与exosomes混合培养24h后迁移能力的影响;观察HUVECs与exosomes混合培养24h后管腔样结构形成变化。结果:各浓度exosomes均具有促进HUVECs细胞增殖作用,且以时间剂量依赖性促进HUVEC细胞增殖(P<0.01);200μg/ml exosomes处理HUVECs细胞24小时,HUVEC细胞的迁移能力提高,差异具有显著性(P<0.01),且能显著提高HUVECs体外管腔形成能力,差异具有统计学意义(P<0.05)。结论:MDA-MB-231细胞源exosomes以时间剂量依赖的方式促进HUVECs增殖,且可显著增强HUVECs的迁移能力及其体外管腔形成能力。Objective：To observe the effect of exosomes derived from MDA-MB-231 cell line on proliferation,immigration and capillary-like tube formation of Human Umbilical Vein Endothelial cells.Method：Exosomes were purified by serial ultracentrifugation and sugar density ultracentrifugation,MTT assay was used to observe the effect of exosomes on proliferation of HUVECs;HUVECs were treated with exosomes for 24 h,the change of cell migration was detected by Transwell chamber method.The capillary-like tube formations by HUVECs were observed.Result：MTT result showed that the concentration range of exosomes significantly increased HUVECs proliferation in a concentration-and time-dependent manner（P0.01）;It may significantly enhance the migration of HUVECs after treated with 200 μ/ml exosomes for 24 h（P0.01）,and may significantly promoted the capability of capillary-like tube formation of HUVECs（P0.05）.Conclusion：Exosomes derived from MDA-MB-231 cell line significantly increased HUVECs proliferation in a concentration-and time-dependent manner and it may promote the migration and the capability of capillary-like tube formation of HUVECs.

关 键 词：乳腺癌细胞株 EXOSOMES 脐静脉内皮细胞株 增殖 迁移 管腔形成 

分 类 号：R730.23[医药卫生—肿瘤]