云南马铃薯品种(系)块茎中的龙葵素含量测定  

Determination of the Content of Solanine in Potato Tuber in Yunnan

作　　者：黄红苹[1] 郭华春[1] 王琼[1] 沈词专[1] 周晨[1] 

机构地区：[1]云南农业大学农学与生物技术学院薯类作物研究所,昆明650201

出　　处：《中国农业科学》2011年第7期1512-1518,共7页Scientia Agricultura Sinica

基　　金：国家现代农业产业技术体系建设专项资金(nycytx-15);云南省科技攻关项目(2009BB010)

摘　　要：【目的】测定云南省不同品种(系)马铃薯块茎中龙葵素含量,研究机械损伤、变绿、长芽、遭受虫蛀和局部霉烂等因素对龙葵素含量的影响,为马铃薯食用安全提供理论依据。【方法】采用双溶剂超声提取龙葵素粗品,HPLC法测定云南省131个品种(系)马铃薯块茎中龙葵素的含量。【结果】云南主栽品种的龙葵素含量均不超过10 mg.(100 g)-1 FW,在安全范围内;地方品种也仅有3.12%超过安全值;而新品系超过安全值比例为12.12%,最高的达48.59 mg.(100 g)-1 FW;有30.30%在10—20 mg.(100 g)-1 FW。机械损伤、虫蛀、表皮变绿、发芽或局部霉烂等会使马铃薯块茎的龙葵素含量增加。【结论】云南马铃薯主栽和地方品种的龙葵素含量较低,而新引进和选育的品种(系)超出安全值的比例较高,这可能与品种遗传背景有关。随着中国马铃薯遗传资源引进与利用的扩大,应将新品种的龙葵素含量作为马铃薯品质评价的必测指标,以确保马铃薯的食用安全。【Objective】 The varieties of potato in Yunnan and the solanine content in mechanical damaged potato,green potato,germinating potato,worm eaten potato and local midew potato were determined in a study to provide a theoretical basis for food safety.【Method】 Crude solanine was extracted with ultrasonic solvent pairs extraction method.HPLC was used to determine solanine contents of 131 varieties of potato in Yunnan.【Result】The solanine content of main cultivar in Yunnan was no more than 10 mg·（100 g）^-1 FW.Only 3.12% of the old varieties were over the safe value.But the solanine content of 12.12% of varieties were over the safe value,even the highest content in some of them reached 48.59 mg·（100 g）^-1 FW.Just in 30.30% the varieties,the content was in 10 to 20 mg·（100 g）^-1 FW.The solanine content of mechanical damaged potato,green potato,germinating potato and local midew potato increased.【Conclusion】 The main and local varieties of potato in Yunnan province have lower solanine content,while the solanine content in new varieties is higher than the safe level.This might be related to the genetic background.With the introduction and utilization of the potato genetic resources,the solanine content of new varieties must be as one of the quality indicators to ensure the food safety of the table potato.

关 键 词：云南 马铃薯 龙葵素 超声提取法 HPLC 

分 类 号：S532[农业科学—作物学]