宫颈永生化细胞和癌细胞的胞浆差异蛋白组学研究  

Comparative proteomics analysis of cytoplasmic proteins of immortalized cervical cell and cervical cancer cell

作　　者：宋大萍[1] 赵涌[2] 

机构地区：[1]绵阳市第三人民医院病理科,绵阳621000 [2]重庆医科大学基础学院病理学教研室,重庆400016

出　　处：《重庆医科大学学报》2010年第12期1859-1862,共4页Journal of Chongqing Medical University

摘　　要：目的:对HPV-16阳性的H8细胞株(宫颈上皮永生化细胞)和Caski细胞株(宫颈癌细胞)胞浆蛋白进行双向电泳和质谱分析,寻找与宫颈癌相关的特异性差异蛋白质。方法:采用双向凝胶电泳(2-dimensronal gel electrophoresis,2-DE)和基质辅助激光解吸飞行时间串联质谱(Matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry,MALDI-TOF-MS/MS)以及SWISS-PROT数据库,对H8和Caski细胞胞浆蛋白进行比较,找出差异蛋白质。结果:通过对宫颈永生化细胞和癌细胞胞浆蛋白2-DE图谱比较和质谱分析,初步鉴定出6个差异蛋白,它们分别为STK3、TRAF5、S100PI、KKb、CDK6、TAF2,其中S100P、IKKb、CDK6、TAF2、TRAF5这5种蛋白质在宫颈癌细胞胞浆中高表达,STK3蛋白质在宫颈癌细胞胞浆中表达缺失。结论:初步发现STK3、TRAF5、S100PI、KKb、CDK6、TAF2蛋白质在宫颈癌前病变和宫颈癌细胞胞浆中存在差异表达,通过对其功能的了解研究为宫颈癌前病变向癌转变机制的探讨、特异性肿瘤标志物的寻找提供实验依据。Objective：To identify differentially expressed protein spots of cytoplasm from H8 cells（human immortalized endocervical cell） and Caski（cervical cancer cell） associated with cervical carcinogenesis by two-dimensional electrophoresis（2-DE） analysis.Methods：The cytoplasmic proteins of H8 cell and Caski cell were separated by means of two-dimensional gel electrophoresis,then the selected differential protein spots were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry（MALDI-TOF-MS） and SWISS-PROT database.Results：6 differentially expressed protein spots were successfully identified,among which,S100P,IKKb（Inhibitor of nuclear factor kappa-B kinase subunit beta）,CDK6（Cell division protein kinase 6）,TAF2（Transcription initiation factor TFIID subunit 2）,TRAF5（TNF receptor-associated factor 5） were significantly up-regulated in cytoplasmic proteins in Caski cell.STK3（Serine/threonine-protein kinase 3） deletion was found in cytoplasmic proteins in Caski cell.Conclusion：The differential expressions of proteins of cytoplasm may be valuable for studying mechanisms of cervical carcinogenesis,or as diagnostic markers and therapeutic targets for cervical cancer.

关 键 词：H8细胞 CASKI细胞 差异蛋白质 2-DE 基质辅助激光解吸飞行时间串联质谱 

分 类 号：R361.3[医药卫生—病理学]