HLA-A02基因型与中国高发区肝细胞癌的密切关联  

Association of HLAA02 genotype with hepatocellular carcinoma in a prevalent area of China

作　　者：明利华[1,2] 朱源荣 王晓宏 吴志远[1,2] 孙宗棠 

机构地区：[1]中国协和医科大学中国医学科学院肿瘤研究所分子肿瘤学国家重点实验室 [2]中国江苏省启东肝癌研究所

出　　处：《中国免疫学杂志》1999年第7期305-307,310,共4页Chinese Journal of Immunology

摘　　要：目的：探讨ＨＬＡＡ０２型及其亚型与启东肝细胞癌的相关性。方法：用ＰＣＲＳＳＰ法分析启东肝细胞癌和正常人中ＨＬＡＡ０２型及其亚型的分布。结果：１０３例经病理诊断的肝细胞癌中５７例为ＨＬＡＡ０２型，其比例达到５５％，显著高于启东正常人群ＨＬＡＡ０２型３２％（３３／１０２）的比例，Ｐ＜０．００１。同时揭示包括ＨＬＡＡ０２型在内的ＨＬＡＡ位点的等位基因多为杂合型。启东肝细胞癌ＨＬＡＡ０２型中Ａ０２０１亚型所占的比例稍高于正常人群，但没有显著差异。分析比较表明，启东肝细胞癌中ＨＬＡＡ０２型的相对集中与当地肝癌中ｐ５３基因２４９密码子突变的高频度发生并没有关联。结论：在乙肝病毒和黄曲霉毒素均为危险因子的启东地区，ＨＬＡＡ０２型与肝细胞癌密切相关。Objective: The association of HLAA02 genotype and its subtypes with hepatocellular carcinoma (HCC) was investigated in a prevalent area, Qidong of China. Methods: The HLAA02 genotype were studied by using PCRSSP. Results: 57 of 103 HCC were found to be of HLAA02 genotype. In contrast, 33 of 102 normal people of Qidong were identified to be of HLAA02. The difference is statistically highly significant ( P <0.001). The HLAA locus alleles were found to be mostly heterozygous. The distribution of A02 subtypes was similar to that of normal peoples. 249 codon mutation status of p53 gene of HCC was identified by PCR and HaeIII RFLP analysis, confirmed by DNA sequencing. The results showed that the 249 codon mutation frequency of p53 gene was not associated with the A02 genotype. Conclusion: In an area where HBV and aflatoxin were risk factors, a close association of HLAA02 genotype with hepatocellular carcinoma was identified. This genotype clustering was not associated with the unique 249 codon hotspot mutation of the p53 gene.

关 键 词：HLA-A02 肝细胞癌 P53基因 点突变 

分 类 号：R735.703[医药卫生—肿瘤]