海南坡鹿MD2 cDNA的克隆及其生物信息学分析  

Cloning and Bioinformatics Analysis of MD2 cDNA from Hainan Eld's deer

作　　者：杜丽[1] 谢少林[1] 成鹰[1] 张晓茹[1] 匡文华[1] 焦寒伟[1] 张冬琳[1] 郝永昌[1] 雷明[1] 刘涛[1] 王凤阳[1] 

机构地区：[1]海南大学农学院海南省热带动物繁育与疫病研究重点实验室(筹)海口市动物基因工程重点实验室,海南海口570228

出　　处：《热带生物学报》2011年第1期1-5,共5页Journal of Tropical Biology

基　　金：国家自然科学基金资助项目(30560021)

摘　　要：以海南坡鹿外周血白细胞总RNA为模板,利用RT-PCR技术,获得海南坡鹿MD2全长cDNA,并对其进行较系统的生物信息学分析,内容包括序列特征分析、同源性分析、编码蛋白质理化性质及结构预测。结果表明:海南坡鹿MD2的CDS序列长度为486 bp,编码160个氨基酸,与黄牛、人、欧洲兔、褐家鼠的核苷酸序列同源性分别为98.55%,77.43%,78.47%和74.74%。该基因编码的蛋白质相对分子质量约为19×103,理论等电点为8.18。对编码蛋白的分子结构预测发现,该蛋白为非跨膜蛋白,其二级结构中,α-螺旋占16.25%,β-折叠片占38.13%,无规则卷曲占45.63%。Myeloid differential protein-2（MD2） cDNA of Hainan Eld＇s deer（Cervus eldi hainanus） was amplified by RT-PCR and the sequences were analyzed.The bioinformatics analysis results indicated that MD2 cDNA ORF contained a 486 bp encoding 161 amino acids;compared to yellow cattle,human,rabbit and mouse,the nucleotide sequence homology of MD2 were 98.55%,77.43%,78.47% and 74.74%,respectively;relative molecular weight was 19 000,pI was 8.18.The molecular structure prediction results suggested that the protein was non-transmembrane protein,which contained 16.25% α-helix,38.13% β-meander,45.63 % random coil.

关 键 词：海南坡鹿 MD2 克隆 生物信息学分析 

分 类 号：Q786[生物学—分子生物学]