反胶束体系中纤维素酶降解纤维素的研究  

Study on Enzymatic Degradation of Cellulose in Reverse Micellar System

作　　者：毕红艳[1] 李晶[1] 杨淑娟[1] 孔长青[1] 孙艳芝[1] 张洪林[1] 

机构地区：[1]曲阜师范大学化学与化工学院,山东省曲阜市273165

出　　处：《曲阜师范大学学报（自然科学版）》2011年第2期74-78,共5页Journal of Qufu Normal University(Natural Science)

基　　金：山东省自然科学基金(Y2006B13)

摘　　要：为减小酶与表面活性剂之间的静电作用,提高酶的活性,在丁二酸二异辛酯磺酸钠(AOT)/异辛烷反胶束体系中加入了正辛醇,并用微量量热法研究了AOT/正辛醇/异辛烷反胶束体系中正辛醇浓度(c),含水量(wo),pH及温度(T)对纤维素酶降解纤维素的影响.结果表明,正辛醇的加入确实有利于纤维素酶活性的提高,而在上述反胶束体系中纤维素酶降解纤维素的最佳条件是c=0.11mol.L-1,wo=3.1,pH=5.01,T=315.57 K.To reduce the electrostatic interaction between surfactant and enzyme,and to enhance the enzyme activity,n-octanol was added to the diocryl sulfosuccinate sldium salt（AOT）/iso-octane reverse micellar system.In this paper the influences of the concentration of n-octanol（c）,the molar ratio of water to surfactan（wo）,pH and temperature（T） on enzymatic degradation of cellulose in AOT/n-octanol/iso-octane reverse micellar system have been investigated using microcalorimetric method.The results obtained indicate that the addition of n-octanol can enhance the cellulase activity,and the optimal conditions for enzymatic degradation of cellulose in the reverse micellar system mentioned above was c=0.11 mol·L-1,wo=3.1,pH=5.01,T=315.57 K.

关 键 词：反胶束 纤维素酶 纤维素 微量量热法 对比进度法 

分 类 号：O642.3[理学—物理化学]