高产乙酸乙酯酵母的产酯条件研究  

Researching the Ester Production Conditions of the high Production Ethyl Acetate Yeast

作　　者：刘源才 郭圣祥 李锐利[2] 陈茂彬[2] 方尚玲[2] 

机构地区：[1]劲牌有限公司,湖北435100 [2]湖北工业大学生物工程学院发酵工程省部共建教育部重点实验室,湖北武汉430068

出　　处：《食品与发酵科技》2011年第2期22-24,共3页Food and Fermentation Science & Technology

基　　金：湖北省自然科学基金(2008CDB063);2009年博士科研启动基金BSQD0910;发酵工程省部共建教育部重点实验室开放基金(2009KFJJ01)

摘　　要：从酿酒酒曲中分离筛选出一株产乙酸乙酯能力较强的酵母菌Y14,在玉米糖化液中总酯产量为2.152g/L。菌株通过VITEK-32微生物鉴定仪鉴定为Hansenula anomala。通过单因素试验,确定了该酵母菌株最佳产酯培养基是小麦糖化液,而且静止培养比振荡培养产酯高40%。再选取温度、糖度、pH值、发酵时间、酒精添加量五因素设计五因素四水平的正交试验,得出该酵母菌株最佳产酯条件为:糖度为8°,pH为5,温度为28℃,培养时间是4d,酒精添加量为2%。在该优化产酯方案下Y14的产酯量为:4.812g/L。A higher yield of ethyl acetate yeast Y14 was selected from starter-making for liquor-making,the production volume of total ester was 2.152g/L in corn saccharification mash.the strains was identified as Hansenula anomala.Firstly through the single factor test to study the effects of diffident culture mediums and diffident culture methods on ester-producing,the results showed that the best ester-producing culture medium for Y1 yeast was the wheat saccharification mash,the ester yield of static culture was 40% higher when compared with shaking culture.Selected several factors to carry on the orthogonal test once more,the result indicated that the optimization plan was as followed： brix was 8°,pH was 5,temperature was 25℃,culture time was 4 days,and ethyl alcohol was 2%,total ester production of Y14 was 4.812g/L in this conditions.

关 键 词：产酯酵母 乙酸乙酯 产酯条件 正交试验 

分 类 号：Q93-3[生物学—微生物学] TS201.3[轻工技术与工程—食品科学]