亚铁血红素的酶法制备及其保护工艺  

Enzymatic preparation and protection technology of heme

作　　者：庄红[1] 朱媛媛[1] 张婷[1] 陈乐群[1] 刘静波[1] 

机构地区：[1]吉林大学军需科技学院,长春130062

出　　处：《吉林大学学报（工学版）》2011年第3期869-875,共7页Journal of Jilin University:Engineering and Technology Edition

基　　金：'十一五'国家科技支撑计划项目(2007BAD55B06);吉林省科技发展计划项目(20090570)

摘　　要：选用碱性和风味酶对血红蛋白进行复合酶解制取亚铁血红素,分别利用单因素、正交和回归试验优化水解条件,且采用单一抗氧化剂、还原剂及抗氧化剂/还原剂组合保护亚铁血红素中的二价铁。试验结果表明:在碱性蛋白酶底物质量分数为7%,酶质量分数为1%,pH值为8,酶解2h,二次酶解风味酶pH值为6.5,酶质量分数为2%,继续酶解2h,亚铁血红素得率可达到11.18mg/mL;保护剂亚硫酸钠(0.03%)/L-抗坏血酸棕榈酸酯(0.03%)的亚铁保护性能最强,可使亚铁血红素得率达到12.60mg/mL,提高了11.27%。Alkaline and flavorzyme were used for the enzymolysis of hemoglobin to obtain heme. Single factor, orthogonal and regression experiment were employed to optimize hydrolysis conditions, which affect the two enzymes. Single antioxidant, single reductant, composite antioxidants, antioxidant and reductant composition were added to protect ferrous iron. The optimal conditions were： first, the suhstrate concentration of 7 %, alkaline concentration of 1%, pH of 8, hydrolysis time 2 hours; then flavor enzyme concentration of 2 %, pH of 6 %, another two hours hydrolysis time; the yield of heme of 11.18% was achieved. The best ferrous iron protective performance was achieved with protective agents anhydrous sodium sulfite of 0.03 % and 1-ascorbyl palmitate 0.03 %; the yield of ferroprotoporhyrin reached 12. 60 mg/ml, which was raised by 11.27%.

关 键 词：食品加工技术 亚铁血红素 酶解参数 复合保护 

分 类 号：TS251.93[轻工技术与工程—农产品加工及贮藏工程]