生存素反义寡核苷酸对人脑胶质瘤U87细胞株MMP-2及TIMP-2表达的影响  

Effect of survivin ASODN transfection on the expression of MMP-2 and TIMP-2 in human U87 glioma cells

作　　者：高广中[1] 黄俊星[2] 肖蔚[3] 戴桂红[3] 于鸿[3] 

机构地区：[1]泰州市人民医院神经外科,江苏泰州225000 [2]泰州市人民医院肿瘤科,江苏泰州225000 [3]泰州市人民医院病理科,江苏泰州225000

出　　处：《江苏大学学报（医学版）》2011年第2期97-101,F0002,共6页Journal of Jiangsu University：Medicine Edition

基　　金：江苏省医学重点人才基金资助项目(RC2007036);泰州市311工程人才基金资助项目(201036)

摘　　要：目的:研究生存素(survivin)反义寡核苷酸(antisense oligodeoxynucleotide,ASODN)对人脑胶质瘤U87细胞基质金属蛋白酶-2(matrix metalloproteinase-2,MMP-2)及基质金属蛋白酶组织抑制因子-2(tissue inhibitor of metallo-proteinase-2,TIMP-2)表达的影响,探讨生存素ASODN治疗胶质瘤的可行性。方法:经脂质体介导将生存素ASODN转染人脑胶质瘤细胞株U87,用荧光显微镜检测转染效率,用逆转录-聚合酶链反应(RT-PCR)、蛋白质印迹法检测转染细胞生存素、MMP-2及TIMP-2表达改变。结果:荧光显微镜检测结果显示生存素ASODN可成功转染U87细胞。与对照组相比,转染组细胞生存素、MMP-2、TIMP-2 mRNA和蛋白的表达均显著降低。结论:生存素ASODN转染可有效抑制U87细胞生存素基因的表达,并降低其MMP-2、TIMP-2表达,生存素ASODN可能用于胶质瘤基因治疗。Objective： To investigate the effect of survivin antisense oligodeoxynucleotide（ASODN） transfection on the expression of matrix metalloproteinase-2（MMP-2） and tissue inhibitor of metalloproteinase-2（TIMP-2） in human brain glioma cell line U87 and to provide experimental foundation for survivin ASODN gene therapy for glioma.Methods： Survivin ASODN was transfected into U87 cells through lipofectin.The rate of transfection was detected by fluorescence microscopy.The expressions of survivin,MMP-2 and TIMP-2 in transfected cells were detected by RT-PCR and Western blot.Results： Survivin ASODN was successfully transfected into U87 cells.After the transfection,RT-PCR and Western blot analysis revealed that the expressions of survivin,MMP-2 and TIMP-2 mRNA and their proteins of antisense group were decreased significantly,compared with the control group.Conclusion： The transfection of survivin ASODN could effectively down-regulate the expressions of survivin,MMP-2 and TIMP-2 in U87 cell.Survivin ASODN may provide potential gene therapy for glioma.

关 键 词：生存素 反义寡核苷酸 基质金属蛋白酶-2 基质金属蛋白酶组织抑制因子-2 人脑胶质瘤细胞 

分 类 号：R739.41[医药卫生—肿瘤]