乳饮料中烟酸和烟酰胺的液相色谱检测方法  

Dairy Products Nicotinic Acid and Nicotinamide Liquid Chromatography Detection Methods

作　　者：王春天[1] 戴洋洋 闫磊[1] 张延超[1] 马文红[1] 郑维君[1] 

机构地区：[1]天津市乳品食品监测中心,天津300381

出　　处：《食品研究与开发》2011年第4期148-151,共4页Food Research and Development

摘　　要：建立一种测定乳饮料中烟酸和烟酰胺含量的高效液相色谱法。样品经乙晴萃取后进样,采用C18 100 mm×2.1 mm色谱柱分离,流动相为甲醇、异丙醇、庚烷磺酸钠。紫外检测;A-R261 nm,RSD为0.56%-0.82%（n=5）,标准曲线r=0.999 8,平均回收率分别为98.2%,烟酸最低检出限为6.25μg/100 g,烟酰胺最低检出限为12.5μg/100 g。To establish a milk drink determination of nicotinic acid and nicotinamide by HPLC method.The sample was extracted with acetonitrile,C18 100 mm ×2.1 mm column with mobile phase of methanol,isopropanol,heptane sulfonate.UV detection;A-R261 nm,RSD is 0.56 %-0.82 %（n=5）,the correlation coefficients were more than 0.999 8,the recoveries were 98.2 %.The detection limit of nicotinic acid was 6.25 μg/100 g,the detection limit of Nicotinamide was 12.5 μg/100 g.

关 键 词：超高压液相色谱(UPLC) 烟酸 烟酰胺 乳饮料 

分 类 号：TS275.4[轻工技术与工程—农产品加工及贮藏工程]