体外香菇多糖对顺铂抑制胃癌细胞增殖的促进作用  

Lentinan enhances cisplatin-mediated inhibition of cell proliferation in human gastric cancer cell line SGC-7901

作　　者：伍海鹰[1] 陈一明[1] 林龙[1] 林友刚[1] 邱庆安[1] 刘宁[1] 

机构地区：[1]海南省人民医院普外科,海南省海口市570311

出　　处：《世界华人消化杂志》2011年第4期344-348,共5页World Chinese Journal of Digestology

摘　　要：目的:研究体外香菇多糖(Lentinan)对多药耐药基因表达的影响和促进顺铂抑制胃癌细胞增殖的作用.方法:分别用顺铂(Cisplatin)、香菇多糖和顺铂联合香菇多糖处理SGC-7901胃癌细胞,将其分为4组:对照组(Con组),香菇多糖组(Len组),顺铂组(Cis组)和顺铂联合香菇多糖组(L+C)组.应用RT-PCR检测多药耐药基因MDR1、MRP1和LRP基因mRNA表达;应用CCK-8试剂盒检测Con组和药物处理组前后的胃癌细胞增殖状态.结果:正常SGC-7901胃癌细胞中多药耐药基因MDR1、MRP1和LRP均表达mRNA,香菇多糖能显著降低多药耐药基因表达而对细胞增殖无明显影响;顺铂明显增加多药耐药基因表达,同时抑制细胞增殖(P<0.05);香菇多糖联合顺铂作用后,MDR1和MRP1基因表达完全受到抑制,LRP表达显著降低,细胞增殖速度明显低于Con组、Len组和Cis组,差异具有统计学意义(10d:0.54vs1.90,1.88,0.92,均P<0.05).结论:香菇多糖联合顺铂后因抑制多药耐药基因表达而显著增强顺铂的抑制细胞增殖作用.AIM：To investigate whether lentinan enhances cisplatin-mediated inhibition of cell proliferation in human gastric cancer cell line SGC-7901 and to explore its effect on the expression of multidrug resistance genes.METHODS：SGC-7901 cells were divided into four groups：untreated cells（control group）,those treated with lentinan alone（lentinan group）,those treated with cisplatin alone（cisplatin group）,and those treated with both lentinan and cisplatin（lentinan ＋ cisplatin group）.RTPCR was applied to detect the mRNA expression of MDR1,MRP1,and LRP in SGC-7901 cells.The proliferation of SGC-7901 cells was detected using the Cell Counting Kit-8.RESULTS：High expression of multidrug resistance genes MDR1,MRP1 and LRP was detected in untreated SGC-7901 cells.Treatment with lentinan significantly decreased the mRNA expression of multidrug resistance genes but had no effect on cell proliferation（P 0.05）.Cisplatin treatment lessened cell proliferation and promoted the expression of multidrug resistance genes.Treatment with lentinan ＋ cisplatin completely suppressed the mRNA expression of MDR1 and MRP1 and significantly decreased LRP expression and cell proliferation compared with the control group,lentinan group,and cisplatin group（10 d：0.54 vs 1.90,1.88,0.92,all P 0.05）.CONCLUSION：Lentinan combined with cisplatin can signif icantly inhibit the expression of multidrug resistance genes and strongly enhance cisplatin-mediated inhibition of the proliferation of SGC-7901 cells.

关 键 词：香菇多糖 顺铂 胃癌 多药耐药基因 细胞增殖 

分 类 号：R735.2[医药卫生—肿瘤]