休哈塔假丝酵母HDYXHT-01利用木糖生产乙醇的发酵工艺优化  

Optimization of xylose fermentation for ethanol production by Candida shehatae HDYXHT-01

作　　者：葛菁萍[1] 刘国明[1] 杨晓峰[1] 孙红兵[1] 凌宏志[1] 平文祥[1] 

机构地区：[1]黑龙江大学生命科学学院微生物黑龙江省高校重点实验室,哈尔滨150080

出　　处：《生物工程学报》2011年第3期404-411,共8页Chinese Journal of Biotechnology

基　　金：国家高技术研究发展计划(863计划)(No.2007AA100702-6);国家自然科学基金(No.31070446);黑龙江省教育厅重点项目(No.11551z011);黑龙江省科技攻关重大项目(No.GA07B401-6);哈尔滨市科技创新人才研究专项资金项目优秀学科带头人(No.RC2010XK002028);黑龙江大学高层次人才(创新团队)支持计划(No.hdtd2010-17)资助~~

摘　　要：采用Plackett-Burman(PB)方法和中心组合设计(Central composit design,CCD)对休哈塔假丝酵母Candida shehatae HDYXHT-01利用木糖发酵生产乙醇的工艺进行优化。PB试验设计与分析结果表明:硫酸铵、磷酸二氢钾、酵母粉和接种量是影响木糖乙醇发酵的4个关键因素,以乙醇产量为响应目标,采用CCD和响应面分析法(Response surface methodology,RSM),确定了木糖乙醇发酵的最佳工艺为:硫酸铵1.73 g/L、磷酸二氢钾3.56 g/L、酵母粉2.62 g/L和接种量5.66%,其他发酵条件为:木糖80 g/L,MgSO4.7H2O 0.1 g/L,pH 5.0,培养温度30℃,装液量100 mL/250 mL,摇床转速140 r/min,发酵时间48 h,在该条件下发酵液中乙醇产量可以达到26.18 g/L,是优化前的1.15倍。Plackett-Burman（PB） design and central composite design（CCD） were applied to optimize of xylose fermentation for ethanol production by Candida shehatae HDYXHT-01.The PB results showed that（NH4）2SO4,KH2PO4,yeast extract and inoculum volume were the main affecting factors.With ethanol productivity as the target response,the optimal fermentation was determined by CCD and response surface analysis（RSM）.The optimal fermentation conditions were（NH4）2SO4 1.73 g/L,KH2PO4 3.56 g/L,yeast extract 2.62 g/L and inoculum volume 5.66%.Other fermentation conditions were xylose 80 g/L,MgSO4.7H2O 0.1g/L,pH 5.0 and 250 mL flask containing 100 mL medium and cultivated at 30 °C for 48 h and the agitation speed was 140 r/min.Under this fermentation conditions,ethanol productivity was 26.18 g/L,which was 1.15 times of the initial.

关 键 词：休哈塔假丝酵母 木糖 乙醇 响应面分析法 

分 类 号：TS262.2[轻工技术与工程—发酵工程]