一株产细菌素物质芽孢杆菌的筛选和鉴定  

Screening and Identification of Bacteriocin-like Substance Producing Bacillus

作　　者：李俊峰[1] 李红芳[1] 段孝辉 宿烽[1] 田智刚 

机构地区：[1]青岛科技大学化工学院,青岛266042 [2]烟台出入境进出口检验检疫局,烟台264000 [3]晋中市环保局榆次区分局,晋中030600

出　　处：《科学技术与工程》2011年第12期2644-2648,共5页Science Technology and Engineering

基　　金：中国烟草总公司山东省公司科技计划项目(KN172)资助

摘　　要：从石油污染的土壤中筛选出一株能产生细菌素类物质的芽孢杆菌,命名为SLY—3。该菌株分泌的活性物质抑菌活性好,对细菌主要是革兰氏阳性菌、霉菌都有抑制作用。从表型、生理生化反应及16S rDNA序列比对方面进行分析,最终确定菌株SLY—3为枯草芽孢杆菌(Bacillus subtilis)。培养基初始pH为7.0,28℃振荡培养24 h后,发酵产物抑菌活性最高。One Bacillus strain named SLY—3 producing bacteriocin-like substance（BLS） was screened from the soil polluted by oil.The G＋ bacteria and molds were effectively inhibited by BLS produced by strain SLY—3.With the analysis of colony morphology,physiological and biochemistry experiments and 16S rDNA gene sequence,the strain SLY—3 was identified as Bacillus subtilis.The BLS secreted by Bacillus subtilis SLY—3 has the highest inhibitory activity when it is cultured for 24 h at initial pH 7.0 and 28 ℃.

关 键 词：细菌素 枯草芽孢杆菌 鉴定 抑菌活性 

分 类 号：Q939.124[生物学—微生物学]