儿茶素对脑胶质瘤细胞U251增殖的抑制作用及机制初探  

Inhibitory effect of catechins on proliferation of human glioma U251 cells

作　　者：梁丽琴[1] 金刚[2] 党建章[2] 代建国[2] 陈卫平[1] 张燕[2] 

机构地区：[1]江西农业大学食品科学与工程学院,南昌市330045 [2]深圳职业技术学院应用化学与生物技术学院,518055

出　　处：《实用医学杂志》2011年第9期1526-1528,共3页The Journal of Practical Medicine

摘　　要：目的:通过体外实验初步研究儿茶素对脑胶质瘤细胞U251增殖的抑制作用及其诱导凋亡的机制。方法:选取8个不同梯度浓度的儿茶素进行甲基噻唑蓝(MTT)实验,观察儿茶素的半致死浓度;倒置显微镜下观察细胞形态,台盼蓝拒染法及Hoechst33342荧光染色检测细胞凋亡,用流式细胞术检测儿茶素对细胞周期的影响。结果:儿茶素对U251细胞的半抑制浓度为62.25μg/mL,儿茶素作用后U251细胞出现凋亡小体,阻滞于S期,Hoechst33342检测表明细胞凋亡明显。结论:儿茶素明显抑制U251细胞增殖,阻滞细胞周期于S期。儿茶素具有开发为治疗脑胶质瘤药物的可能性。Objective To investigate the inhibitory effect of catechins on proliferation of human glioma U251 cells in vitro and illustrate the potential mechanism. Methods MTT assay was performed to determine the semi-lethal concentration of catechins for U251 cells. Trypan blue staining and Hoechst33442 fluorescent staining were conducted to detect U251 cell apoptosis. U251 cell morphology was observed under inverted microscope. Cell cycle status of catechins treated U251 cells was determined by the flow cytometry. Results The semi-lethal concentration of catechins for U251 cells was 62.25μg/mL. Apoptotic bodies appeared in catechins treated U251 cells, and U251 cell cycle was arrested at S stage. The apoptotic U251 cells increased obviously in a dosedependent and time-dependent manner after catechins treatment. Conclusion Catechins can efficiently inhibit U251 cell proliferation, and it is a promising drug candidate for glioma.

关 键 词：脑肿瘤 神经胶质瘤 儿茶素 U251细胞 细胞凋亡 

分 类 号：R739.4[医药卫生—肿瘤]