Ⅱ型ZTC1+1天然澄清剂在巴西菇多糖提纯中的应用  

The Effect of Ⅱ Type ZTC1+1 Natural Clarifying Agents Used for Agaricus brasiliensis Polysaccharide Purification

作　　者：史德芳[1] 高虹[1] 周明[1] 杨德[1] 关健[1] 陈学玲[1] 何建军[1] 

机构地区：[1]湖北省农业科学院农产品加工与核农技术研究所/湖北省农业科技创新中心农产品加工分中心/国家食用菌加工技术研发分中心,武汉430064

出　　处：《湖北农业科学》2011年第6期1250-1254,共5页Hubei Agricultural Sciences

基　　金：国家星火计划项目(2008GA760005);湖北省农业科技创新中心资助项目(2007-620-001-03)

摘　　要：在考察Ⅱ型ZTC1+1天然澄清剂对巴西菇多糖提纯工艺影响的过程中,用单因素试验研究了提取原液稀释倍数、澄清剂用量、澄清剂絮凝温度对多糖损失率和蛋白脱除率的影响,进一步以多糖纯度为考察指标,利用响应面分析法优化各因素及其相互作用后获得的最佳工艺条件为提取原液稀释倍数7.20,澄清剂用量7.44%,澄清剂絮凝温度57.23℃,此时,巴西菇多糖纯度为62.78%。In the purification of polysaccharides in Agaricus brasiliensis by Ⅱ type ZTC1＋1 natural clarifying agents,single factor experiment was adopted to study the effects of dilution fold of extract,the amount of Ⅱ type ZTC1＋1 added and flocculation temperature on the protein removal rate and loss rate of polysaccharides.Furthermore,the optimization of the various factors and their interation was analyzed by response surface method through determining the purity of polysaccharides.The optimum experimental conditions were as follows： the dilution multiple was 7.20,the adding amount of natural clarifying agent（i.e.B and A） to the concentrated solution was 7.44%,the flocculation temperature was 57.23℃.Under these conditions,the purity of polysaccharides was 62.78%.

关 键 词：巴西菇 多糖 II型ZTC1+1天然澄清剂 脱蛋白 提纯 

分 类 号：S646.11[农业科学—蔬菜学] R284.2[农业科学—园艺学]