基因芯片技术检测宫颈病变中HPV感染的临床研究  

Clinical Analysis of Human Papillomavirus in Paraffin-embedded Cervical Lesion Tissues by Gene Chip Technology

作　　者：彭敏[1] 宋春林[1] 王夷黎[1] 周东华[1] 秦艳[1] 

机构地区：[1]广东省佛山市妇幼保健院,528000

出　　处：《国际检验医学杂志》2011年第5期583-584,共2页International Journal of Laboratory Medicine

摘　　要：目的应用基因芯片技术检测宫颈疾病中人乳头瘤病毒(HPV)感染的基因型别及分布特点探讨其临床意义。方法回顾性分析经病理确诊的119例宫颈癌前病变及宫颈癌中应用基因芯片技术检测23种HPV基因型的感染情况。结果总共检测出23种HPV基因型中的19种基因型,其中HPV52和HPV16居感染的前两位,在CIN3及宫颈癌中排在前6位的基因型是HPV16,33,58,31,52,68,宫颈癌中排在第1位的是HPV16。HPV双重或多重感染在宫颈病变各级别中均占有一定的比例。结论导致宫颈高级别病变及宫颈癌的仍然是高危型HPV,但存在地理差异。HPV16感染仍然是宫颈癌的最大威胁,多重感染的问题值得关注。Objective Gene chip technology was used to detect the genotyping and distribution characteristics of human papillo mavirus （HPV） in cervical disease. The clinical significance of gene chip technology was discussed. Methods 119 pathologically di agnosed samples with cervical cancer and precancerous pathological changes were retrospectively analyzed to detect the infection sit uations of 23 kinds of HPV types, using gene chip technology. Results 19 of the all 23 kinds of HPV types were detected, headed by the HPV52 and HPV16 types. The 6 most popular types in the infection CIN3 and cervical cancer were HPV16, 33, 58, 31, 52 and 68. HPV16 is the most popular type in cervical cancer. Conclusion The high risk HPV types was the reason to cause the high-level cervical lesions and cervical cancer. However, there was geographic variation. The infection caused by HPV16 type was still the most dangerous threat. More attention should be paid to multiple infection in cervical cancer.

关 键 词：宫颈上皮内瘤变 宫颈癌 基因型 人乳头瘤病毒 

分 类 号：R737.33[医药卫生—肿瘤]