麻风树叶多糖的分离纯化及组成分析  

Extraction,purification and component analysis of polysaacharide from Jatropha curcas L.leaves

作　　者：许子竞[1] 林翠梧[1] 黄艳伟[1] 姚先超[1] 蒋炳丽[1] 

机构地区：[1]广西大学化学化工学院,广西南宁530004

出　　处：《食品工业科技》2011年第5期84-86,89,共4页Science and Technology of Food Industry

基　　金：广西科技攻关项目(桂科攻0480005);广西大学科学技术研究重点基金资助项目(2003ZD05)

摘　　要：使用DEAE-纤维素、活性炭脱色和凝胶过滤色谱从麻风树叶粗多糖中纯化得到了两种多糖组分(JLP-3-1和JLP-3-2),经过高效凝胶渗透色谱分析,它们为均一多糖,相对分子质量为3.2968×106、1.8152×106Da左右。IR和HPLC分析表明,两组分(JLP-3-1和JLP-3-2)单糖主要是鼠李糖、果糖、半乳糖,也含有少量半乳糖醛酸。Two ingredients（JLP-3-1 and JLP-3-2）were obtained from crude polysaccharides which were extracted from Jatropha curcas L.leaves by GPC Chromatography,decoloring with active carbon and DEAE-cellulose.Their homogeneities were verified by HPGPC,the molecular wight of JLP-3-1 and JLP-3-2 were about 3.2968×106,1.8152×106Da.The units of monosaccharide were identified by HPLC as D-galacturonic acid,D-rhamnose,D-fructose and D-galactose.IR and HPLC analysis showed two ingredients（JLP-3-1 and JLP-3-2）contained a little galacturonic acid.

关 键 词：麻风树叶多糖 分离纯化 凝胶渗透色谱 HPLC IR 

分 类 号：TS201.2[轻工技术与工程—食品科学]