从发酵液中高效提取L-缬氨酸的工艺研究  

Study on L-Valine Efficient Extraction Technology from the Fermentation Broth

作　　者：李新涛[1] 徐庆阳[1] 冯宁[1] 谢希贤[1] 陈宁[1] 

机构地区：[1]天津科技大学生物工程学院,天津300457

出　　处：《生物技术通讯》2011年第2期229-233,共5页Letters in Biotechnology

基　　金：国家科技重大专项(2008ZX09401-05);天津市科技支撑计划重点项目(08ZCKFSH01900)

摘　　要：目的:利用有机膜过滤和离子交换法分离提取发酵液中的L-缬氨酸。方法:通过有机膜过滤,除去发酵液中的菌体及蛋白,滤液浓缩结晶获得L-缬氨酸产品,通过离子交换法从结晶母液中回收部分L-缬氨酸。结果:确定了有机微滤膜和超滤膜去除发酵液中菌体蛋白和色素的操作条件;确定了采用离子交换法提取L-缬氨酸的操作条件:选择732强酸性阳离子树脂,料液pH值为3.0左右,用0.4 mol/L的氨水以1.0 mL/min的速度洗脱,L-缬氨酸的收率为89.2%。结论:通过有机膜过滤和离子交换法分离提取发酵液中的L-缬氨酸,可以提高提取收率和产品质量。Objective：Using organic membrane filtration and ion exchange to extract L-valine from the fermentation broth.Methods：The bacteria and proteins in the fermentation broth were removed through organic membrane filtration,the filtrate was concentrated to obtain L-valine crystal products,the part of L-valine was further extracted from the crystal liquor by ion exchange.Results：The optimum conditions of organism membrane filtration,decolour and abstracton of L-valine were determined.732 resin was the optimal resin for L-valine abstraction among the different kinds of resins.The optimal conditions of adsorption were as follow：dosage liquor pH3.0,and the elution of L-valine was carried out at the rate of 1.0 mL/min using 0.4 mol/L ammonia water as eluant.The recovery of L-valine was up to 89.2%.Conclution：L-valine extraction yield and product quality was improved by the way of organic membrane filtration and ion exchange.

关 键 词：L-缬氨酸 发酵 提取 离子交换 

分 类 号：TQ922[轻工技术与工程—发酵工程] Q503[生物学—生物化学]