N^+离子注入诱变选育纤维素酶高产菌  

Screening of High-yield Cellulase-producing Strains by N^+ Implantation Mutation

作　　者：陈阿娜[1] 汤斌[1] 刘标[1] 

机构地区：[1]安徽工程大学生物与化学工程学院,安徽芜湖241000

出　　处：《激光生物学报》2011年第2期171-174,共4页Acta Laser Biology Sinica

基　　金：安徽省高校自然科学基金重点资助项目(KJ2007A018);安徽工程科技学院青年科研基金项目(2007YQ001zd)

摘　　要：纤维素酶产生菌Rhizopus sp.TC1经过N^+离子注入、紫外线、5-氟尿嘧啶(5-Fu)等诱变剂诱变,选育得到突变株Rhizopus sp.TC1653,发酵终点时滤纸酶活力(FPA)和羧甲基纤维素酶活力(CMC)分别为22.6IU/g、224.7IU/g干物质,较出发菌株分别提高了276.7%和214.3%。突变株经传10代培养,任意2代间酶活力差值均在10%内,表明该菌株产酶性能稳定,可作为后继实验出发菌株。突变株在培养基上能够快速生长,发酵96h即达到酶活高峰期,较绿色木霉酶活高峰期提前48h,缩短了发酵周期。该实验结果表明,经N^+离子注入等方式诱变选育的突变株具有孢子萌发率高,同步性好,代谢速度快等优点。Cellulase-producing strain Rhizopus sp. TC1 was subjected to mutation involving treatment of N ^＋ implantation, UV followed by 5-Fu; successive mutant Rhizopus sp. TC1653 showed enhanced cellulase production. The FPA and CMC case of mutant TC1653 was 22.6 and 224.7 IU per gram of dry medium respectively at the end of fermentation, which was increased by 3.77 folds and 3.14 folds respectively compared to that of TC1. Ten times subculturing in- dicated that the mutant TC1653 had good genetic stability and character of cellulase production. TC1653 showed rapid growth on solid-state medium and its cellulase activities were highest at 96 hours. Fermentation time shortened to for 48 hours compared to that of Trichoderma virid. The results showed that mutant TC1653 had some advantages under the mutation caused by N^ ＋ implantation.

关 键 词：纤维素酶 N^+离子注入 传代培养 发酵周期 

分 类 号：Q689[生物学—生物物理学] Q939.5