Fasudil对EAE小鼠脊髓内细胞ROCK-Ⅱ表达的影响  

The effect of Fasudil on expression of ROCK-Ⅱ in spinal cords in experimental autoimmune encephalomyelitis mice

作　　者：李雁翔[1,2] 李艳花[1] 尉杰忠[1] 郭敏芳[1] 肖保国[1] 马存根[1,2] 

机构地区：[1]山西大同大学脑科学研究所,037008 [2]山西医科大学第一临床医学院神经内科,太原030001

出　　处：《中国医疗前沿》2011年第6期47-49,44,共4页China Healthcare Innovation

基　　金：国家自然科学基金(编号:81070957);山西省回国留学人员科研资助项目(编号:2008-86);山西大同大学博士科研启动经费(编号:2008-b-21)

摘　　要：目的探讨Fasudil对实验性自身免疫性脑脊髓炎(EAE)小鼠脊髓内细胞及血管内皮细胞ROCK-Ⅱ表达的影响。方法雌性C57BL/6小鼠随机分为佐剂组、EAE组和Fasudil干预组。EAE和Fasudil组小鼠用髓鞘少突胶质细胞糖蛋白35-55(MOG35-55)进行免疫,佐剂组小鼠以生理盐水代替MOG35-55作为EAE的对照。Fasudil干预组于免疫后第7d腹腔给予Fasudil(50mg/kg/d),连续14d。佐剂组和EAE组给与等量生理盐水作为对照。免疫后隔天观察各组小鼠临床评分和体重变化。在免疫4周后处死动物,取小鼠脊髓腰膨大处,进行HE和免疫荧光染色,观察各组小鼠脊髓的病理变化与ROCK-Ⅱ的表达情况。结果 Fasudil能够改善EAE的临床症状(P<0.05),减轻炎性细胞浸润(P<0.05),同时减少EAE小鼠脊髓及其血管内皮细胞ROCK-Ⅱ的表达。结论 EAE小鼠脊髓及其血管内皮细胞ROCK-Ⅱ的表达明显上调,推测可能与炎性细胞浸润和疾病的发生发展有关。Fasudil可以改善EAE的临床症状,可能与脊髓及其血管内皮细胞ROCK-Ⅱ降低,及由此引起的炎性细胞浸润减少有关。Objective To investigate the effect of Fasudil on expression of ROCK-Ⅱ in spinal cords and vascular endothelial cells in experimental autoimmune encephalomyelitis mice.Methods Female C57BL /6 mice were randomly divided into adjuvant group,EAE group and Fasudil-treated group.EAE group was set up as Fasudil-treated group control,while adjuvant group was performed as EAE group control.Fasudil was intraperitoneally injected in the dosage of 50mg·kg-1·d-1 on day 7 post-immunization（p.i.） for 14 consecutive days.Mice in EAE and adjuvant group were injected with saline.Clinical signs were evaluated and body weight was recorded every other day.Mice were sacrificed on days 28-30p.i.,and the pathological changes in spinal cords were detected by HE staining.The expression of ROCK-Ⅱ in spinal cords was detected by immunofluorescence staining.Results The clinical symptom and the inflammatory infiltration of spinal cords in Fasudil-treated mice were significantly ameliorated compared with those of EAE mice（P0.05）.After the intervention of Fasudil,the expression of ROCK-Ⅱin spinal cords was decreased.Conclusion The expression of ROCK-Ⅱ was significantly upregulated in EAE mice,while treatment of Fasudil improved the clinical symptoms of EAE mice,which perhaps relates to the inhibition of ROCK-Ⅱand the decrease of inflammatory infiltration.

关 键 词：多发性硬化 实验性自身免疫性脑脊髓炎 RHO/ROCK信号通路 FASUDIL 

分 类 号：R365[医药卫生—病理学] R744[医药卫生—基础医学]