产复合酶枯草芽孢杆菌QLB6的激光诱变育种  

Breeding of a Mutant Strain of Bacillus subtilis QLB6 with Higher Proteolytic Activity by He-Ne Laser Irradiation

作　　者：宋鹏[1] 黄亮[2] 陈亮[2] 

机构地区：[1]河南科技大学农学院,河南洛阳471003 [2]河南工业大学生物工程学院,河南郑州450001

出　　处：《食品科学》2011年第9期222-224,共3页Food Science

基　　金：河南科技大学博士科研启动基金项目(09001379)

摘　　要：以产复合酶枯草芽孢杆菌QLB6为出发菌株,利用氦-氖(He-Ne)激光进行诱变育种。通过计算存活率和正突变率,确定激光诱变育种的照射剂量。通过透明圈筛选和酶活力测定,筛选获得1株编号为QLB6F的菌株,其纤维素酶、蛋白酶、淀粉酶酶活力相对于出发菌株分别提高219%、38%和71%。进行30代遗传稳定性实验,证明它具有良好的遗传稳定性,无回复突变。In this study,Bacillus subtilis QLB6 that had been isolated from aged tobacco leaves and been found to have higher proteolytic activity was mutagenized by means of He-Ne laser irradiation.The optimum irradiation dose was determined based on survival rate and positive mutation rate.Based on transparent circle and proteolytic acivity,a target strain was obtained and named QLB6F.The strain exhibited an increase of 219%,38% and 71% respectively in cellulose,protease and amylase activities when compared to QLB6.After the 30th passage,the genetic stability of the mutant strain remained excellent and no reverse mutation was observed.

关 键 词：枯草芽孢杆菌 复合酶 HE-NE激光 遗传稳定性 

分 类 号：S852.616[农业科学—基础兽医学] S335.29[农业科学—兽医学]