冬虫夏草液体深层发酵培养基的优化工艺研究  

Optimizing on Liquid Culture Media of Cordyceps Sinensis Mycelia

作　　者：尚才人 白兰芳[2] 王金主[3] 郑军科 

机构地区：[1]浙江方圆检测集团股份有限公司,浙江杭州310013 [2]中国计量学院,浙江杭州310018 [3]济南大学,山东济南250022

出　　处：《山东化工》2011年第4期72-74,共3页Shandong Chemical Industry

摘　　要：采用正交分析方法对冬虫夏草液体深层发酵培养基配方进行了优化,结果表明,冬虫夏草生长最适液体培养基配方为:土豆20%、牛肉膏0.08%、蛋白胨0.2%、KH2PO40.15%、MgSO4.7H2O 0.15%、蔗糖1.5%、葡萄糖2.5%;温度为23℃,转速为130r/min,初始pH值为7,培养周期4d,菌丝体生物量产率比优化前提高了2倍。The liquid culture media of cordyceps sinensis mycelia was optimized by means of orthogonal analysis. The results showed that the optimal medium for the myeelia was 20% potato, 0. 08% beef extraxt 0.2% peptone, 0.15 % KH2PO4, 0. 15 % MgSO4 · 7H2O,1.5% sucrose,2.5% glucose and pH natural. In the cultural condition of 23 ℃, 130r/min and 4 d, the mycelia biomass could gain 19. 5 g/L , which was as 2 times higher than that before optimization.

关 键 词：正交分析 冬虫夏草 菌丝体 液体培养基 优化 生物量 

分 类 号：S567.35[农业科学—中草药栽培]