高效液相色谱法测定南、北五味子中4种木脂素含量  

Determination of Lignins in Schisandra sphenanthera and Schisandra chinensis by High Performance Liquid Chromatography

作　　者：阴冠秀[1] 杜冰[1] 鲁旺旺[1] 华洋林 唐建 杨公明[1] 

机构地区：[1]华南农业大学食品学院,广东广州510642 [2]无限极(中国)有限公司,广东广州510665

出　　处：《食品科学》2011年第10期218-221,共4页Food Science

摘　　要：目的:采用高效液相色谱法同时测定17种不同产地的五味子中五味子醇甲、五味子醇乙、五味子甲素和五味子乙素含量。方法:采用Diamonsil C18(250mm×4.6mm,5μm)柱,甲醇-水(73:27,V/V)作流动相,流速1.0mL/min,检测波长254nm。结果:五味子醇甲、五味子醇乙、五味子甲素和五味子乙素含量测定方法的线性关系良好,线性范围为0.01～0.08mg/mL,相关系数R2>0.9990;RSD分别为0.86%、0.37%、0.19%和0.15%。结论:该方法精密度、重现性和分离效果好,分析快速准确,适合于该药材中五味子醇甲、五味子醇乙、五味子甲素和五味子乙素含量的测定;不同产地的4种木脂素成分均存在一定的差别,北五味子中五味子醇甲、五味子醇乙和五味子乙素的含量稍高,而五味子甲素含量却稍低,南五味子恰与之相反。Objective： To establish a HPLC method for the determination of schisandrin,schisandrol B,deoxyschizandrin and schisandrin B in Schisandra sphenanthera Rehd.et Wils.and Schisandra chinensis（Turcz.） Baill.from 17 growth areas.Methods：The chromatographic separation was carried out on Diamonsil C18（250 mm× 4.6 mm,5 μm） column using methanol-water（73：27,V/V） as the mobile phase at a flow rate of 1 mL/min.The detection wavelength was 250 nm.Results： A good linear relationship was achieved with a correlation coefficient in the range of 0.01 to 0.08 mg/mL（R2 = 0.9990） and a RSD of 0.86%,0.37%,0.19% and 0.15%,respectively.Conclusion： The developed HPLC method reveals a good separation efficiency,reproducibility and accuracy.This method can be used for determining schisandrin,schisandrol B,deoxyschizandrin and schisandrin B in herbs.Higher amounts of schisandrin,schisandrol B and schisandrin B are present in Schisandra chinensis than in Schisandra sphenanthera.

关 键 词：南五味子 北五味子 高效液相色谱 木脂素 

分 类 号：R284.1[医药卫生—中药学]