利用响应面法优化ε-聚赖氨酸发酵培养基  

Medium optimization of ε-polylysine production by response surface analysis

作　　者：张超[1] 张克峰[1] 侯书国[1] 马永山[1] 

机构地区：[1]山东建筑大学市政与环境工程学院,山东济南250101

出　　处：《微生物学通报》2011年第5期641-646,共6页Microbiology China

摘　　要：采用响应面法对白色链霉菌ZC7发酵合成ε-聚赖氨酸的培养基进行优化研究。采用Plackett-Burman法对8个因素进行了筛选,结果表明,葡萄糖、酵母膏和硫酸铵的浓度对ε-聚赖氨酸产量影响较大。用最陡爬坡试验及Box-Behnken设计进一步优化,利用Design-Expert软件进行二次回归分析,得到各因素的最佳浓度为:葡萄糖37.22 g/L,酵母膏6.9 g/L,硫酸铵6.55 g/L。在此优化条件下ε-聚赖氨酸的产量达到8.11 g/L,较单因素试验最高值提高24.6%。In order to enhance the concentration of ε-polylysine（ε-PL） with Streptomyces albulus ZC7,response surface methodology were used to optimize the fermentation medium.A Plackett-Burman de-sign was used to evaluate the influence of eight factors firstly.Results showed that the concentration of glucose,yeast extract and（NH4）2SO4 played important roles in influencing the content of ε-PL.Then,the path of steepest ascent and the Box-Behnken design were adopted for further optimization,and the optimum concentration levels and the relationships among these factors was found out by quadratic re-gression model equation with Design-Expert statistic methods,the optimal concentration of the vari-ables were determined as： glucose 37.22 g/L,yeast extract 6.9 g/L,（NH4）2SO4 6.55 g/L.Under such conditions,the concentration of ε-PL was increased to 8.11 g/L,which was 24.6% higher than the maximum value in the single factor tests.

关 键 词：白色链霉菌 Ε-聚赖氨酸 优化 响应面 

分 类 号：TQ921[轻工技术与工程—发酵工程]