中国不同地区蜂胶的抑菌性研究  

Antimicrobial Activity of Chinese Propolis from Different Areas

作　　者：龚上佶[1] 郭夏丽[1] 罗丽萍[1] 付宇新[2] 徐元君[1] 陈滨[1] 

机构地区：[1]南昌大学生命科学与食品工程学院,江西南昌330031 [2]江西省林业科学院,江西南昌330032

出　　处：《北京工商大学学报（自然科学版）》2011年第2期23-27,共5页Journal of Beijing Technology and Business University:Natural Science Edition

基　　金：国家自然科学基金项目(30700105;31071551);江西省青年科学家(井冈之星)培养计划资助项目(S00590)

摘　　要：蜂胶具有显著的抑菌性,常被做成药品或保健品.为了解中国蜂胶的抑菌性,本实验以采自中国23个省的29种蜂胶(根据采集地气候区域分为5类)为研究对象,分别提取获得蜂胶醇提物(EEP),并测定了EEP对枯草杆菌、金黄色葡萄球菌、产气杆菌、大肠杆菌、变形杆菌、毛霉、青霉、曲霉、根霉的抑菌圈大小和最小抑菌浓度,比较不同气候区域蜂胶的抑菌性差异.结果表明,EEP对革兰氏阳性菌、革兰氏阴性菌和真菌均有明显的抑菌性,平均抑菌圈直径分别为:9.88mm±1.47 mm、8.75 mm±1.56 mm和10.01 mm±0.65 mm,EEP对真菌的抑菌性最强,其次为革兰氏阳性菌,最弱为革兰氏阴性菌;9个菌种中,EEP对变形杆菌的抑菌性最差,对根霉的抑菌性最好;不同气候区域蜂胶的EEP抑菌性没有显著差异.Propolis has strong antimicrobial activity,and is often used as drugs or health products.Twenty nine different propolis from 23 provinces of China were selected and studied in this paper,which were further classified into five groups according to the climate zone where collected.The size of inhibition zone and minimum inhibitory concentration（MIC） of 9 different strains（Staphylococcus aureus,Bacillus subtilis,Aerobacter aerogenes,Escherichia coli,Proteus,Penicillium,Aspergillus,Mucor,Rhizopus） by the ethanol extract of different propolis（EEP） were measured.The results showed that EEP had good antimicrobial activity against Gram-positive bacteria,Gram-negative bacteria and fungi.The average inhibition zone diameter of Gram-positive bacteria,Gram-negative bacteria and fungi were 9.88mm±1.47mm,8.75mm±1.56mm and 10.01mm±0.65mm,respectively.EEP exhibited the highest antimicrobial activity against fungi,while the lowest activity against Gram-negative bacteria.The results indicated that the antimicrobial activity of EEP had no significant correlation with their climate zone.

关 键 词：蜂胶 醇提物 抑菌性 

分 类 号：TS202.1[轻工技术与工程—食品科学] TS201.3[轻工技术与工程—食品科学与工程]