一株可降解几丁质的Bacillus sp D23的分离和筛选  

ISOLATION AND SCREENING A BACILLUS SP D23 STRAIN WITH HIGH CHITINASE ACTIVITY

作　　者：缑敬轩[1,2] 董文宾[1,2] 曾桥[1,2] 

机构地区：[1]浙江温州轻工研究院,浙江温州325003 [2]陕西科技大学生命科学与工程学院,陕西西安710021

出　　处：《陕西科技大学学报（自然科学版）》2011年第2期32-35,共4页Journal of Shaanxi University of Science & Technology

基　　金：温州市科技计划项目(H200900332);陕西科技大学自然科学基金项目(ZX10-19)

摘　　要：采用透明圈法和DNS法测发酵液酶活相结合的办法,从土壤样品中筛选到一株降解几丁质能力强的菌株D23.该菌株在分离平板上产生的透明圈和菌落直径的比值为9,发酵液的酶活为0.75 U/mL.经形态特征及16S rDNA序列分析,确定D23属于芽孢杆菌属.In order to screen a new strain with high chitinase activity,the samples around Wei River area were collected,and with method of studying the transparent circle on the plate,and further confirmed by studying the activity of fermentation broth by DNS method,a new strain（D23） with high chitinase activity was isolated.Data shows that the diameter ratio of transient circle and the colony circle in the plate containing colloidal chitin and colony is about as high as 9.Morphology study and sequencing of the 16S rRNA information indicated that strain D23 was Bacillus sp.

关 键 词：几丁质酶 筛选 酶活力 

分 类 号：Q939.9[生物学—微生物学]