脂肪酶产生菌地霉7203菌株的分离和诱变  

ISOLATION AND MUTAGENSIS OF LIPASE PRODUCER GEOTRICHUM SP.7203 STRAIN

作　　者：陶文沂 邬显章 向瑞春 吴燕 

机构地区：[1]无锡轻工业学院

出　　处：《微生物学报》1990年第3期216-222,共7页Acta Microbiologica Sinica

摘　　要：从76个土样中分离获得271株脂肪酶产生菌,其中7203菌株产酶活性最高,从其形态特征确定属地霉属。对该菌株脂肪酶合成受油脂、脂肪酸及某些结构类似物的影响进行了考察,发现琥珀酸钠对该菌株生长及产酶有明显的负控制作用。经诱变筛选药物耐性变异株,使产酶能力有较大的提高。对改良株的发酵条件及特征作了初步试验,并对有关机理进行讨论。From 76 soil samples 271 strains wereisolated.Of which strain 7203 was the bestone.By its morphological characteristics itwas identified as Geotrichum sp..The effectsof fats,fatty acids and some structural an-alogues in the medium on enzyme producti-vity were studied.It was found that sodiumsuccinate had evidently a negative control ac-tion on microbial growth and lipase produc-tivity.After mutagensis some resistance mu-tants were selected which had higher lipaseproducing ability.The characteristics of themutants and the fermentation optimizationwere studied.Some relative mechanism werealso discussed.

关 键 词：脂肪酶 地霉 分离 诱变 

分 类 号：TQ925.9[轻工技术与工程—发酵工程]