蒸汽爆破玉米秸秆酶解动力学  

Kinetics of enzymatic hydrolysis of steam-explosion pretreated corn straw

作　　者：任省涛[1,2] 程可可[2] 宋安东[1] 张建安[2] 

机构地区：[1]河南农业大学生命科学学院,郑州450002 [2]清华大学核能与新能源技术研究院,北京100084

出　　处：《生物工程学报》2011年第4期592-597,共6页Chinese Journal of Biotechnology

基　　金：国家高技术研究发展计划(863计划)(No.2007AA100702);国家重点基础研究发展计划(973计划)(No.2011CB707406)资助~~

摘　　要：为了掌握蒸汽爆破玉米秸秆的酶解特性,研究了不同底物浓度、酶浓度、温度对反应速率的影响。运用米氏方程对酶解动力学过程进行拟合,结果表明,纤维素酶对该玉米秸秆的水解反应在反应前3 h符合一级反应,可用米氏方程对其进行拟合。在转速为120 r/min、酶浓度为1.2 FPU/mL、pH 5.0、温度为45℃时米氏常数Km为11.71 g/L,最大反应速率Vm为1.5 g/（L.h）。确立了包括底物浓度、酶浓度、温度在内的酶解动力学模型,该模型适合温度为30℃-50℃。In order to learn the enzymatic hydrolysis characteristics of steam-explosion pretreated corn straw by cellulase,the effects of substrate concentration,cellulase concentration and temperature were determined.The kinetics of the hydrolysis reaction could be described with the Michealis-Menten equation,and the hydrolysis reaction obeyed the classical first-order reaction rate in the first three hours.In the condition of 45 °C and pH 5.0 and the stirring rate 120 r/min,the Michealis constant（Km） and maximum rate（Vm） for 1.2 FPU/mL of cellulase were 11.71 g/L and 1.5 g/（L？h）.The kinetic model,including the parameters such as substrate concentration,enzymatic concentration and temperature,was suit for the hydrolysis reaction under the temperature range from 30 °C-50 °C.

关 键 词：米氏方程 玉米秸秆 酶解 动力学 纤维素酶 

分 类 号：S216.2[农业科学—农业机械化工程]