Sildenafil and FDP-Sr attenuate diabetic cardiomyopathy by suppressing abnormal expression of myocardial CASQ2, FKBP12.6, and SERCA2a in rats  

作　　者：Yu-si CHENG De-zai DAI Hui JI Qi ZHANG Yin DAI 

机构地区：[1]The Faculty of Pharmacy, China Pharmaceutical University, Nanjing 210009, China [2]The Bioengineering and Pharmaceutical Faculty, Nanjing University of Technology, Nanjing 210009, China

出　　处：《Acta Pharmacologica Sinica》2011年第4期441-448,共8页中国药理学报（英文版）

基　　金：Acknowledgements This project was supported by the National Natural Science Foundation of China （No 30873112）.

摘　　要：Aim： To study whether calcium-modulating proteins CASQ2, FKBP12.6 and SERCA2a participate in diabetic cardiomyopathy, and whether the beneficial actions of testosterone, sildenafil or fructose diphosphate Sr （FDP-Sr） in the treatment of diabetic cardiomyopa-thy result from suppressing these molecules. Methods- Fifty male Sprague-Dawley （SD） rats were divided into five groups. Except for the normal group （non-diabetic）, the other four groups were injected with streptozotocin （STZ, 60 mg/kg, ip） to induce diabetes. Four weeks after STZ injection, the four groups received sildenafil （12 mg·kg^-l·d^-1, ig, for 4 week）, FDP-Sr （200 mg/kg, ig, for 4 week）, testosterone propionate （4 mg·kg^-l·d^-1, sc, for 4 week）, or no treatment, respectively. Results： In the diabetic rats, blood glucose, free fatty acids, triglycerides, total cholesterol, and low-density lipoprotein cholesterol （LDL-C） were significantly increased, while high-density lipoprotein cholesterol （HDL-C） was significantly reduced, as compared to the non-diabetic rats. Cardiac dysfunction and myocardial hypertrophy of the diabetic rats were associated with increased mRNA and protein expression of iNOS, OBRb, and PKCε, while expression of CASQ2, SERCA2a, and FKBP12.6 was significantly down-regulated. Sildenafil and FDP-Sr, but not testosterone, significantly attenuated the biomarker abnormalities, without changing the metabolic abnormalities. Conclusion： CASQ2, FKBP12.6 and SERCA2a were down-regulated in diabetic cardiomyopathy. Sildenafil and FDP-Sr, but not testoster-one, attenuated the cardiac dysfunction in diabetic cardiomyopathy, without changing the metabolic abnormalities, which may results from inhibiting oxidative and inflammatory cytokines and improving calcium homeostasis.Aim： To study whether calcium-modulating proteins CASQ2, FKBP12.6 and SERCA2a participate in diabetic cardiomyopathy, and whether the beneficial actions of testosterone, sildenafil or fructose diphosphate Sr （FDP-Sr） in the treatment of diabetic cardiomyopa-thy result from suppressing these molecules. Methods- Fifty male Sprague-Dawley （SD） rats were divided into five groups. Except for the normal group （non-diabetic）, the other four groups were injected with streptozotocin （STZ, 60 mg/kg, ip） to induce diabetes. Four weeks after STZ injection, the four groups received sildenafil （12 mg·kg^-l·d^-1, ig, for 4 week）, FDP-Sr （200 mg/kg, ig, for 4 week）, testosterone propionate （4 mg·kg^-l·d^-1, sc, for 4 week）, or no treatment, respectively. Results： In the diabetic rats, blood glucose, free fatty acids, triglycerides, total cholesterol, and low-density lipoprotein cholesterol （LDL-C） were significantly increased, while high-density lipoprotein cholesterol （HDL-C） was significantly reduced, as compared to the non-diabetic rats. Cardiac dysfunction and myocardial hypertrophy of the diabetic rats were associated with increased mRNA and protein expression of iNOS, OBRb, and PKCε, while expression of CASQ2, SERCA2a, and FKBP12.6 was significantly down-regulated. Sildenafil and FDP-Sr, but not testosterone, significantly attenuated the biomarker abnormalities, without changing the metabolic abnormalities. Conclusion： CASQ2, FKBP12.6 and SERCA2a were down-regulated in diabetic cardiomyopathy. Sildenafil and FDP-Sr, but not testoster-one, attenuated the cardiac dysfunction in diabetic cardiomyopathy, without changing the metabolic abnormalities, which may results from inhibiting oxidative and inflammatory cytokines and improving calcium homeostasis.

关 键 词：diabetic cardiomyopathy CASQ2 FKBP12.6 SERCA2A SILDENAFIL FDP-Sr testosterone calcium homeostasis 

分 类 号：Q582[生物学—生物化学] T-332[一般工业技术]