Breviscapine attenuates acute pancreatitis by inhibiting expression of PKCα and NF-κB in pancreas  

作　　者：Hong Zhang Cui-Zhu Cai Xiao-Qin Zhang Tao Li Xiao- Yun Jia Bao-Lan Li Liang Song Xiao-Jun Ma 

机构地区：[1]Department of Pathophysiology, Shaanxi University of Traditional Chinese Medicine, Xianyang 712046, Shaanxi Province, China [2]Department of Internal Medicine, Sanya Hospital of Traditional Chinese Medicine, Sanya 572000, Hainan Province, China

出　　处：《World Journal of Gastroenterology》2011年第14期1825-1830,共6页世界胃肠病学杂志（英文版）

基　　金：Supported by Funds of Natural Science of Shaanxi Education, No.05JK176;Natural Science of Shaanxi Province, No.2010JM4023;Natural Science of Xianyang City, No. 2010K14-02(6)

摘　　要：AIM:To study the effect of breviscapine (Bre) on activity of protein kinase Cα (PKCα) and nuclear factor (NF)-κB in pancreas,and the mechanism of Bre attenuating acute pancreatitis (AP). METHODS:One hundred and eight rats were randomly divided into acute necrotizing pancreatitis (ANP) group,Bre group (ANP + Bre group) and sham operation (SO) group,36 rats in each group. ANP model was induced by a retrograde injection of 4% sodium deoxycholate into the bilio-pancreatic duct. Fifteen minutes after the ANP model was induced,the rats in Bre group were intraperitoneally injected with Bre (0.4 mg/100 g body weight or 0.1 mL/100 g body weight). Survival time and mortality of rats were calculated. Serum amylase and malondialdehyde levels were measured,volume of ascites was recorded and morphology of pancreas and lung was evaluated at 1,5 and 10 h,after the ANP model was induced,respectively. Expressions of PKCα and subunit p65 of NF-κB in pancreas were detected by immunohistochemistry and Western blotting. RESULTS:The life span of rats was longer and the mortality was lower in Bre group than in ANP group 13.51 ± 5.46 vs 25.36 ± 8.11 (P < 0.05). The amylase and MDA levels as well as the volume of ascites were lower and the pathological changes in pancreas and lung were less in Bre group than ANP group (P < 0.05),indicating that the pancreatitis is less severe in Bre group than ANP group. The activation of PKCα and NF-κB p65 in pancreas was induced rapidly and reached their peak at 1 h or 5 h after ANP,but their activity in Bre group was significantly inhibited. CONCLUSION:Bre exerts its therapeutic effect on AP by inhibiting the activation of PKCα and NF-κB p65 in pancreas.AIM：To study the effect of breviscapine （Bre） on activity of protein kinase Cα （PKCα） and nuclear factor （NF）-κB in pancreas,and the mechanism of Bre attenuating acute pancreatitis （AP）. METHODS：One hundred and eight rats were randomly divided into acute necrotizing pancreatitis （ANP） group,Bre group （ANP ＋ Bre group） and sham operation （SO） group,36 rats in each group. ANP model was induced by a retrograde injection of 4% sodium deoxycholate into the bilio-pancreatic duct. Fifteen minutes after the ANP model was induced,the rats in Bre group were intraperitoneally injected with Bre （0.4 mg/100 g body weight or 0.1 mL/100 g body weight）. Survival time and mortality of rats were calculated. Serum amylase and malondialdehyde levels were measured,volume of ascites was recorded and morphology of pancreas and lung was evaluated at 1,5 and 10 h,after the ANP model was induced,respectively. Expressions of PKCα and subunit p65 of NF-κB in pancreas were detected by immunohistochemistry and Western blotting. RESULTS：The life span of rats was longer and the mortality was lower in Bre group than in ANP group 13.51 ± 5.46 vs 25.36 ± 8.11 （P 0.05）. The amylase and MDA levels as well as the volume of ascites were lower and the pathological changes in pancreas and lung were less in Bre group than ANP group （P 0.05）,indicating that the pancreatitis is less severe in Bre group than ANP group. The activation of PKCα and NF-κB p65 in pancreas was induced rapidly and reached their peak at 1 h or 5 h after ANP,but their activity in Bre group was significantly inhibited. CONCLUSION：Bre exerts its therapeutic effect on AP by inhibiting the activation of PKCα and NF-κB p65 in pancreas.

关 键 词：BREVISCAPINE Acute pancreatitis Protein kinase Cα Nuclear factor-κB RAT 

分 类 号：Q55[生物学—生物化学] S858.292[农业科学—临床兽医学]