强化乙酰辅酶A供应对裂殖壶菌合成二十二碳六烯酸的影响  

Effect of Reinforcing Acetyl-CoA Supply in Docosahexaenoic Acid Production by Schizochytrium sp.

作　　者：魏萍[1] 任路静[1] 纪晓俊[1] 仝倩倩[1] 冯云[1] 黄和[1] 

机构地区：[1]南京工业大学生物与制药工程学院材料化学工程国家重点实验室,南京210009

出　　处：《中国生物工程杂志》2011年第4期87-91,共5页China Biotechnology

基　　金：江苏省高校科研成果产业化推进项目(JH09-30);教育部新世纪优秀人才资助项目(NCET-09-0157);教育部霍英东教育基金(123014);江苏省六大人才高峰项目(2008-D-63)资助项目

摘　　要：细胞液中乙酰辅酶A的持续供应是脂肪酸高效积累的必要条件。考虑到甲羟戊酸和脂肪酸合成途径共用相同的前体乙酰辅酶A,抑制甲羟戊酸途径可能促使更多的乙酰辅酶A流向脂肪酸合成。通过添加前体物质或/和甲羟戊酸途径酶的抑制剂以强化乙酰辅酶A的供应,即在裂殖壶菌发酵起始或/和后期添加乙酸、发酵起始添加甲羟戊酸途径酶的抑制剂辛伐他汀或柠檬酸、发酵起始同时添加乙酸和辛伐他汀或柠檬酸并考察其对裂殖壶菌合成二十二碳六烯酸(DHA)的影响,结果发现发酵起始同时添加6mmol/L的乙酸和1μmol/L的辛伐他汀时,DHA产量最高,达到13.21g/L,比对照提高了46.61%。In order to achieve efficient fatty acid accumulation in microorganisms,a continuous supply of acetyl-CoA directly in the cytosol of the cell should be satisfied.Considering that acetyl-CoA is the precursor in both the fatty acids synthetic and mevalonate pathway,the mevalonate pathway was inhibited and then more acetyl-CoA might be channeled to the fatty acids synthesis.A method of addition of precursor or/and the inhibitors of mevalonate pathway were used to reinforce acety-CoA supply.Acetate acid is the putative precursor of acetyl-CoA.Simvastatin and citrate acid are the inhibitors of mevalonate pathway.The effects of acetate acid,simvastatin and citrate acid on the performance of docosahexaenoic acid（DHA） production by Schizochytrium sp.were investigated.Including addition of acetate acid at the beginning or/and in the late period of fermentation,addition of simvastatin or citrate acid,the supplementation with acetate acid and simvastatin or citrate acid at the beginning of fermentation.The results showed that the highest of DHA yield reached 13.21g/L by combinations of 6mmol/L acetate acid and 1μmol/L citrate at the beginning of fermentation,which was 46.61% higher than that of control.

关 键 词：乙酰辅酶A 裂殖壶菌 二十二碳六烯酸 

分 类 号：Q819[生物学—生物工程]