响应面法优化重组大肠杆菌产蔗糖异构酶发酵培养基  

Optimization of Fermentation Medium of Sucrose Isomerase by Recombinant Escherichia coli through Response Surface Method

作　　者：汪晨[1] 李莎[1] 徐虹[1] 魏艳[1] 蔡恒[1] 

机构地区：[1]材料化学工程国家重点实验室南京工业大学食品与轻工学院,南京210009

出　　处：《中国生物工程杂志》2011年第4期92-97,共6页China Biotechnology

基　　金：国家自然科学基金(20906050);江苏省自然科学基金(BK2009357);江苏省教育厅自然科学基金(08KJB530004);国家科技支撑计划(2008BAI63B07)资助项目

摘　　要：通过碳氮源的不同浓度对重组大肠杆菌E.coil BL21(DE3)发酵产蔗糖异构酶(SIase)的影响,并借助于数学分析软件Design Expert,结合Plackett-Burman试验设计和中心复合试验设计分析法,对蔗糖异构酶的产生菌进行了发酵培养基的优化研究。实验表明,最佳培养基组分为甘蔗糖蜜10.65 g/L,玉米浆22.22 g/L,NaCl7.57 g/L,MgSO4.7H2O0.52 g/L,KH2PO44.46g/L,优化后的蔗糖异构酶活力达到29.1U/ml,比LB培养基培养重组大肠杆菌(15U/ml),蔗糖异构酶活力提高了94%,与原始菌大黄欧文菌NX-5相比提高了21.4倍(1.3U/ml)。The effects of different concentrations of carbon and nitrogen sources on recombinant E.coli BL21（DE3） on the fermentation of sucrose isomerase were investigated,the culture media of recombinant E.coli producing sucrose isomerase was optimized using Design Expert 7.1.6 software combined with the Plackett-Burman design and central composite design method.The results showed that the optimal media for culture as follows：Cane molasses 10.65 g/L,Corn steep 22.22 g/L,NaCl 7.57g/L,MgSO4·7H2O 0.52g/L,KH2PO4 4.46g/L.It is found that the Sucrose isomerase activity was 29.1U/ml,compared to LB medium culture E.coli（15U/ml）,sucrose isomerase activity increased by 94%,with the original strain increased 21.4 times（1.3U/ml） compared.

关 键 词：蔗糖异构酶 响应面 大肠杆菌 甘蔗糖蜜 乳糖诱导 

分 类 号：Q819[生物学—生物工程]