茯砖茶及其配方对脂肪变性L-02肝细胞中TG含量的影响  

Effects of Fu Brick Tea and Its Formula on the Content of TG in L-02 Steatotic Hepatocytes

作　　者：徐小江[1,2] 黄建安[1,2] 肖力争[1] 袁勇[1,2] 肖文军[1,2] 

机构地区：[1]湖南农业大学园艺园林学院,湖南长沙410128 [2]湖南农业大学茶学教育部重点实验室,湖南长沙410128

出　　处：《茶叶科学》2011年第3期247-254,共8页Journal of Tea Science

基　　金：湖南省重大科技专项"茯茶产业化关键技术研究与示范"(2008FJ1005);国家"十二五"支撑计划资助项目(2011BAD10B00);国家APEC基金资助项目(200847);中国博士后基金资助项目(20070410985)

摘　　要：利用1 mL/L的20%医用脂肪乳剂处理L-02肝细胞48 h复制非酒精性脂肪变性肝细胞,然后加入不同浓度的提取物及配方分别处理24 h和48 h,全自动生化分析仪检测肝细胞内的甘油三酯(TG)水平,探讨茯砖茶提取物和两个配方对脂肪变性L-02肝细胞内TG的影响。结果显示,茯砖茶提取物和配方1与自然恢复组比较均可显著降低脂肪变性肝细胞内TG的水平(P<0.05),而与血脂康组比较无显著差异(P>0.05),显示出茯砖茶和配方1具有广阔的开发应用前景。Using 1 mL/L of 20% medical fat emulsion to process L-02 liver cells for 48 h in order to replicate non-alcoholic fatty degeneration of liver cells,and then adding different concentrations of extracts and its formulas to process separately for 24 h and 48 h,automatic biochemical analyzer detects the level of triglycerides（TG） in liver cells,investigate the effects of Fu Brick Tea extraction and the two formulas on the content of TG in L-02 Steatotic Hepatocytes.Results showed that Fu brick tea extraction and formula 1 can significantly lower TG levels in fatty liver cells（P0.05）,while comparing with the Xue Zhi Kang group,those two groups presented no significant difference（P0.05）.Accordingly,Fu brick tea and formula 1 reflect a wide prospect of development and application.

关 键 词：茯砖茶 脂肪变性 L-02肝细胞 配方 甘油三酯 

分 类 号：TS272.54[农业科学—茶叶生产加工] R972.6[轻工技术与工程—农产品加工及贮藏工程]