噻苯哒唑及其代谢产物在蛋鸡体内的残留分布及消除研究  

Distribution and elimination of thiabendazole and its metabolite residue in laying hens

作　　者：尚楠[1] 郭娟[2,3] 张银[2,3] 王浩[2] 张晶[2] 李青山[1] 邵兵[2] 

机构地区：[1]山西医科大学药学院,太原030001 [2]北京市疾病预防控制中心 [3]南昌大学食品科学与技术国家重点实验室

出　　处：《卫生研究》2011年第3期365-367,374,共4页Journal of Hygiene Research

基　　金：卫生行业科研专项项目(No.200902009)

摘　　要：目的探讨噻苯哒唑及其代谢物5-羟基噻苯哒唑在蛋鸡体内和鸡蛋中的残留分布及消除规律。方法选用30只健康蛋鸡进行试验,每天每只蛋鸡经口喂饲噻苯哒唑100mg,连续给药5天,分别于停药后第1天、第3天、第5天和第7天采集鸡蛋以及肝脏、肌肉、脂肪和心脏等组织制备组织匀浆。样品经乙腈提取,MCX固相萃取柱富集净化,超高效液相-串联质谱法测定噻苯哒唑及其代谢物的残留量。结果蛋鸡经口喂饲噻苯哒唑后,主要组织匀浆中5-羟基噻苯哒唑的含量显著高于噻苯哒唑,鸡蛋中两种药物的残留浓度最高。停药第一天时,组织中的残留水平(噻苯哒唑及5-羟基噻苯哒唑的浓度之和)顺序为肝脏>心脏>肌肉>脂肪。鸡蛋中噻苯哒唑的休药期约为7天。结论初步获得了噻苯哒唑及其代谢产物在蛋鸡组织中的残留水平及消除规律。Objective To explore the distribution and elimination of thiabendazole and its metabolite 5-hydrothiabendazole residues in the hens tissues including liver,muscle,heart,fat,as well as in eggs.Methods Laying hens were orally administred thiabendazole for 5 consecutive days（100mg per hen daily） and then the hens were sacrificed at the times of 1 day,3 days,5 days and 7days after the end of treatment.Eggs,liver,muscle,fat and heart tissues were collected and homogenized.The samples were extracted by acetonitrile,further concentrated and purified by an Oasis MCX cartridge,and then the contents of thiabendazole and 5-hydrothiabendazole in tissue homogenates determinated by liquid chromatography electrospray ionisation tandem mass spectrometry（UPLC-ESI-MS/MS）.Results The major residues in the tissue homogenates was 5-hydroxythiabendazole,with more higher concentrations than thiabendazole.Egg samples presented the large majority of both drug residues.For the tissue homogenates,the total concentrations of thiabendazole and 5-hydrothiabendazole residues followed the order of liverheartmusclefat at 1 day after the treatment.The withdrawal period of thiabendazole for eggs was about 7 days.Conclusion Distribution and elimination of thiabendazole and its metabolite residues in laying hens were primarily studied in this study.

关 键 词：噻苯哒唑 5-羟基噻苯哒唑 蛋鸡 药物残留 休药期 

分 类 号：R155.5[医药卫生—营养与食品卫生学] TS207.53[医药卫生—公共卫生与预防医学]