响应面法优化红树林内生真菌#3895产甘露醇的培养基  

Optimization of culture medium of mannitol-producing from Mangrove endophytic fungus #3895 by response surface methodology

作　　者：陈海燕[1] 张雷[1] 张怀泉[1] 林翠梧[1] 佘志刚[2] 林永成[2] 

机构地区：[1]广西大学化学化工学院,南宁530004 [2]中山大学化学与化学工程学院,广州510275

出　　处：《食品科技》2011年第5期50-53,共4页Food Science and Technology

基　　金：国家"863"计划项目(2006AA09Z422);国家自然科学基金项目(20072058);广西自然科学基金项目(桂科自0728257);广西大学博士科研启动项目(DD040032);广西研究生教育创新计划项目(105930903027)

摘　　要：采用Plackett-Burman设计和响应面设计相结合的方法优化甘露醇发酵培养基组成,利用SAS软件设计实验并分析数据。结果表明,培养基中的葡萄糖、酵母浸膏、培养时间是影响甘露醇产量的主要因素。优化后的培养基组成(g/L)为:葡萄糖45.5,酵母浸膏2.2,蛋白胨2.0,pH7.0,28℃静置培养17d。在此条件下,甘露醇含量达到最大值6.21%,较优化前的4.82%提高了28.8%。Plackett-Burman design and response surface methodology were applied to optimize fermentation medium of mannitol. Experimental design and data analysis were obtained by SAS. The results indicated that mannitol yield was mainly influenced by glucose, yeast extract and culture time. The obtained optimal fermentation medium components（g/L） were glucose 45.5, yeast extract 2.2, peptone 2.0, pH 7.0, and static culture 17 days under the temperature of 28 ℃. Under these conditions, the mannitol content could reach 6.21%, which was 28.8% higher than before.

关 键 词：红树林内生真菌 甘露醇 培养基 优化 响应面分析 

分 类 号：TS201.3[轻工技术与工程—食品科学]