海洋细菌Agarivorans albus NBRC102603琼胶酶的分离纯化  

Isolation and purification of agarase from marine bacteria Agarivorans albus NBRC102603

作　　者：朱启忠[1] 朱慧文[1] 孙艳娜[1] 周晓龙[1] 郝梅[1] 郭振博[1] 

机构地区：[1]山东大学威海分校海洋学院,威海264209

出　　处：《生物加工过程》2011年第3期37-40,共4页Chinese Journal of Bioprocess Engineering

基　　金：国家创新性基金项目(101042239);山东大学科技立项重点项目专项基金(A10010)

摘　　要：利用盐析、分子筛和离子交换等方法对海洋细菌Agarivorans albus NBRC102603分泌的琼胶酶粗酶液进行分离纯化,得到琼胶酶A和琼胶酶B。琼胶酶A纯化倍数为17.51倍,酶比活力为881.82 U/mg;琼胶酶B纯化倍数为16.64倍,酶比活力为838.32 U/mg。纯化的琼胶酶经SDS-PAGE检测,显示为单一条带,其相对分子质量分别为酶A 8.36×104和酶B 3.68×104。Agarases A and B were separated and purified by salting-out,molecular sieve,and ion exchange from fermentation broth of marine bacteria Agarivorans albus NBRC102603.The obtained enzyme agarase A was purified 17.6 folds with specific activity of 881.82 U/mg,while agarase B was purified 16.64 folds with specific activity of 838.2 U/mg.The enzymes were identified to be homogeneous by SDS-PAGE.The molecular masses were 8.36×104 and 3.68×104,respectively.

关 键 词：海洋细菌 琼胶酶 分离 纯化 

分 类 号：Q933.31[生物学—微生物学]