均匀设计法优选菱壳中抗胃癌成分的半仿生提取工艺  

Optimal process for semi-bionic extraction of bioactive compounds against gastric cancer from water chestnut shell by uniform designation

作　　者：宁颖[1] 于海宁[2] 周新妹[3] 王维 蒋风 潘晓燕[3] 沈生荣[1] 

机构地区：[1]浙江大学生物系统工程与食品科学学院,浙江杭州310058 [2]浙江工业大学药学院,浙江杭州310014 [3]嘉兴学院医学院,浙江嘉兴314001 [4]杭州华东医药集团康润制药有限公司,浙江安吉313301

出　　处：《浙江大学学报（农业与生命科学版）》2011年第3期332-337,共6页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：浙江南湖"菱壳抗胃癌活性成分的提取纯化"资助项目(2009AY2032)

摘　　要：以SGC7901胃癌细胞存活率为指标,运用半仿生提取方法回收菱壳中的抗胃癌活性成分,考察提取时间、料液比、胃蛋白酶用量、胰蛋白酶用量对提取物抗胃癌活性的影响,并运用均匀设计法确定其最佳提取工艺.结果表明:菱壳与pH≈2提取液按料液质量体积比1∶10,不加入胃蛋白酶,37℃提取30 min后,离心收集沉淀,添加4%胰蛋白酶,菱壳与pH≈8提取液按料液质量体积比1∶10,37℃提取30 min,可获得最佳抑制效果,细胞存活率仅为45.52%.本实验表明,采用半仿生法提取菱壳中的抗胃癌活性成分是可行的.The viability rate of SGC7901 gastric cancer cells was used as an index,the anti-gastric cancer ingredients from the water chestnut shell were reclaimed by semi-bionic extraction.The effects of extraction time,ratio of water to water chestnut shell,amount of pepsin and trypsin on extraction anti-gastric cancer activity were investigated,and the uniform designation was employed to determine the optimum extraction conditions.The results showed that when 1 g shell powder was added to 10 mL solvent（1∶10 ratio of powder to pH≈2 solvent） in the absence of pepsin,extracting for 30 min at 37 ℃,and then being centrifuged to collect precipitate,adding 10 mL pH≈8 solvent and 4% trypsin,extracting for 30 min at 37 ℃,the extracts had the highest activity against gastric cancer and the cell viability was the lowest,i.e.45.52%.Also,this experiment confirmed that water chestnut shell extracts still had a high activity of inhibiting the growth of gastric cancer cells through semi-bionic extraction.

关 键 词：菱壳 抗胃癌活性 半仿生提取 均匀设计 

分 类 号：TS201.4[轻工技术与工程—食品科学]