γ-聚谷氨酸合成菌株的筛选与优化培养  

Screening a novel γ-polyglutamic acid producing strain and optimization of its culture medium

作　　者：阮文辉[1,2] 杨家志[3] 姚俊[1] 曹新[1] 魏钦俊[1] 鲁雅洁[1] 孙东平[3] 

机构地区：[1]南京医科大学基础医学院,南京210029 [2]山西省医药与生命科学研究院,太原030001 [3]南京理工大学化工学院,南京210094

出　　处：《中国酿造》2011年第5期66-69,共4页China Brewing

基　　金：江苏省高校自然科学研究项目(09KJB530007);山西省科技攻关项目资助

摘　　要：从土壤筛中筛选分离获得1株γ-聚谷氨酸合成菌Bacillus subtilis PGS-1,在富含谷氨酸和葡萄糖的培养基中可大量合成γ-聚谷氨酸,与大多文献报道的微生物合成的γ-聚谷氨酸相比,具有较低的分子量(300ku～400ku)和较窄的分子量分布,可适用于低分子量要求的医药、化妆品和水处理等应用领域,值得深入开发研究。为提高γ-聚谷氨酸的发酵产量,对Bacillus subtilis PGS-1的摇瓶培养基条件进行了响应面优化,确定了影响γ-PGA合成的显著因素依次为谷氨酸、葡萄糖和(NH4)2SO4;在优化条件下,γ-聚谷氨酸产量达26g/L,较优化前提高了44%。A novel γ-polyglutamic acid （γ-PGA） producing strain Bacillus subtilis PGS-1 was isolated from soil, which could produce a large amount of γ-PGA in the medium containing glucose and glutamate. Compared with other reported biosynthetic γ-PGA, a lower molecular weight （300ku -400ku） and a narrow polydispersity endowed γ-PGA derived from Bacillus subtilis PGS-1 a promising application, such as in the drug delivery system, cosmetic and water treatment. To achieve an efficient production of γ-PGA, the flask culture medium was optimized by response surface analysis. The experimental results showed that L-glutamate and glucose were the most important factors to the γ-PGA synthesis, and ammonium sulfate was secondly important. Under this conditions, the yield of γ-PGA reached 26g/L, which increased by 44% compared with γ- PGA yield without optimization.

关 键 词：Γ-聚谷氨酸 枯草芽孢杆菌 筛选 响应面 

分 类 号：Q93-331[生物学—微生物学]