轮枝拟青霉中虫草素的微波提取工艺优化  

Optimization of microwave extraction of cordycepin from Paecilomyces verticillatus

作　　者：李祝[1] 施渺筱[2] 葛永怡[1] 周礼红[1] 陈青[3] 肖洋 刘吴娟[1] 

机构地区：[1]贵州大学生命科学学院,贵州贵阳550025 [2]安顺学院化学系,贵州安顺561000 [3]贵州大学化学与化工学院,贵州贵阳550025 [4]贵州省产品质量检验检测院,贵州贵阳550003

出　　处：《中国酿造》2011年第5期74-76,共3页China Brewing

基　　金：贵州大学引进人才科研基金(贵大人基合字(2008)007号);贵州省教育厅自然科学基金(黔教科2009(0075)号)

摘　　要：采用微波提取轮枝拟青霉发酵菌丝体中的虫草菌素,HPLC法测定提取液中的虫草素含量,在单因素试验的基础上,用正交试验进行工艺参数的优化。优选出的最佳提取工艺为蒸馏水40mL,固液比1∶200,中火(P50)处理3min。在此最佳条件下,轮枝拟青霉中虫草素含量为3.247mg/g,工艺简便可行。Microwave-extraction technology for extracting cordycepin from Paecilomyces verticillatus GZ was studied in this paper. On the basis of single-factor experiments, the orthogonal test was used to optimize the technological parameters. The optimal extraction procedure was obtained as follows ： distilled water 40ml, solid-liquid ratio 1 ： 200 and the time of middle fire power treatment 3min. Under these conditions, the content of eordycepin reached 3. 247mg/g. The technology was simple and feasible and can be used as a reference for eordycepin production.

关 键 词：微波提取 轮枝拟青霉 正交试验 虫草菌素 

分 类 号：O658[理学—分析化学]