四株冰川雪细菌产酶能力的初步研究  

Preliminary Study on Enzymatic Activities of Four Glacial Snow Bacteria

作　　者：张淑红[1,2] 杨红超[1] 刘秀花[1,2] 梁峰[1,2] 焦璐[1] 黄倩[1] 

机构地区：[1]商丘师范学院生命科学系,河南商丘476000 [2]河南省高校生物质降解与气化工程技术研究中心,河南商丘476000

出　　处：《生物技术》2011年第2期80-85,共6页Biotechnology

基　　金：河南省教育厅自然科学研究计划项目(2010B180023)资助

摘　　要：目的:从青藏高原冰川雪中筛选出一菌多酶的菌株。方法:对恢复出的4个细菌,通过平板透明法研究其产淀粉酶、脂肪酶和蛋白酶的特性。结果:LHG-C-9为惟一可以产淀粉酶的菌株,所产脂肪酶活性最高。4个菌株均不产蛋白酶。结论:LHG-C-9最适生长温度为15℃,属于耐冷菌。对该菌所产淀粉酶和脂肪酶的性质进行了初步研究,其随产淀粉酶的最适作用温度为50℃;最佳产酶pH值为7.0,该pH值所产酶活为83.9U/mL;在60℃的高温下温浴10min后酶活为0%。该菌株所产脂肪酶的最适作用温度为20℃;最佳产酶pH值为7.0,该pH值所产酶活为9.2U/mL;50℃温浴lh后酶活力不足34%。Objective： Researches were carried out to screen one bacteria with the ability to produce mutile enzyme from glacial snow on the Tibetan Plateau.Method： The enzymatic activities of four bacteria were analyzed by observing and measuring the clearing zones on the flat plate.Result： LHG-C-9 was the only one to produce amylase.It showed the maximum activity of lipase.No one can produce protease among the 4 strains.Conclusion： The optimal growth temperature of LHG-C-9 was 15℃.It was psychrotrophs.Preliminary study was carried out on characteristics of the amylase and lipase produced by LHG-C-9.The optimum temperature and pH for the amylase activity were 50℃ and 7.0,respectively.The yield of amylase was up to 83.91U/mL on the above pH value.The enzyme lost 100% activity after incubation at 60℃ for 10min.The optimum temperature and pH for the lipase activity were 20℃ and 7.0,respectively.The yield of amylase was up to 9.2U/mL on the above pH value.The enzyme remained 34% activity after incubation at 50℃ for 1h.

关 键 词：冰川雪 细菌 淀粉酶 脂肪酶 蛋白酶 酶学特性 

分 类 号：Q935[生物学—微生物学]