山药中降糖活性成分的筛选与比较  

Study on filtration and comparison of hypoglycemic bioactive components in dioscoreae rhizome

作　　者：何凤玲[1] 叶小利[2] 李学刚[1] 李晓芳[1] 浦东[1] 李平[3] 王小华[3] 冯平[3] 

机构地区：[1]西南大学药学院,重庆400716 [2]西南大学生命科学学院,重庆400715 [3]北碚区中医院,重庆400700

出　　处：《食品工业科技》2011年第6期373-375,共3页Science and Technology of Food Industry

基　　金：重庆市攻关项目(CSTC;2010AC5007);重庆市科委攻关项目(CSTC2010AC5007)

摘　　要：目的:研究山药中多糖、皂苷、黄酮粗提物对HepG2细胞的葡萄糖的消耗作用,对四氧嘧啶诱导的糖尿病小鼠的降糖作用,以此来综合比较三种成分的降糖效果。方法:山药乙醇提取物经(D101)大孔树脂柱吸附乙醇梯度洗脱、分离纯化得到50%含量的总黄酮和60%含量的总皂苷,山药水提物纯化得到多糖,然后分别以HepG2细胞、糖尿病小鼠为模型研究了三种成分的降血糖效果。结论:皂苷可增加单位细胞的葡萄糖消耗量,呈现剂量依赖性。山药原粉、皂苷和黄酮能显著降低糖尿病小鼠的饮水量和血糖水平,其中原粉最明显。推测在山药的降血糖作用中三种成分存在协同效应。Objective：To study the effect of extracts of dioscorea rhizome-polysaccharides,saponins and flavones to HepG2 cell with the subject of consumption glucose affection and to hypoglycemic action effect to the diabetic mice model established intra-abdominal injection of alloxan in order to compare the three components＇ hypoglycemic bioactive.Methods：using the D101 macroporous resin adsorption process for the ethanol extracts of dioscoreae rhizome,elution with different concentration of ethanol.Depurating get 50% flavones content or so,and 60% saponins,and get polysaccharides from extracts of dioscoreae rhizome by water.Then use HepG2 cell and the diabetic mice as model to study three components hypoglycemic affections.Results：It demonstrated that saponins could lead to adding consumption of glucose in unit cell and make model dose-dependent.Dioscoreae rhizome,saponins and flavones could decrease the water drinking volume of diabetic mice and level of glucose.Dioscoreae rhizome was best.It concluded that three components showed synergistic effect among dioscoreae rhizome＇s dhypoglycemic bioactive base on the experiment.

关 键 词：山药多糖 山药皂苷 山药黄酮 HEPG2细胞 糖尿病 

分 类 号：TS201.4[轻工技术与工程—食品科学]