动物组织中类胡萝卜素分析、分布的研究进展  

Process on Analysis and Distribution Research of Carotenoids in Animal Tissues

作　　者：薛峰[1] 李晨[1] 潘思轶[1] 

机构地区：[1]华中农业大学食品科技学院,武汉430070

出　　处：《中国粮油学报》2011年第5期122-128,共7页Journal of the Chinese Cereals and Oils Association

基　　金：国家自然科学基金资助项目(30972044)

摘　　要：对动物组织中类胡萝卜素的分析、分布和类胡萝卜素特异性结合蛋白的研究进行了综述。动物组织中类胡萝卜素的提取主要采用混合有机溶剂提取;分析检测主要采用反相高效液相色谱法和二极管阵列检测器;类胡萝卜素主要由小肠黏膜吸收;脂蛋白被认为参与类胡萝卜素的转运;β-胡萝卜素可经生物转化生成视黄醇;动物各组织对类胡萝卜素表现出选择性吸收,且这种选择性吸收因物种不同而差异显著;类胡萝卜素在吸收过程中相互之间存在交互作用,且这种交互作用主要发生在碳氢类胡萝卜素和含氧类胡萝卜素之间;类胡萝卜素的选择性吸收与交互作用被认为与组织中某种特异性结合蛋白的存在相关。The carotenoids analysis and distribution in animal tissues and the study of carotenoids binding protein were reviewed in this article. Complete extraction of carotenoids from animal tissues was reported by using slightly polar solvents plus non-polar solvents;Reversed Phase High Performance Liquid Chromatography, coupling a photodiode array detector, was most often employed in routine use to analyze the carotenoids;carotenoids were absorbed by the mucosa of the small intestine;lipoproteins played an important role in transportation of carotenoids;the various pathways of β-carotene biotransformation were either known or suspected of occurring in mammalian tissues, and pathways known or proposed were involved in the conversion of β- carotene to retinoids ; selective absorption of carotenoids was found in animal tissues and there were obvious variabilities in the selective absorption of carotenoid among animal species;the interactions between carotenoids were found during the process of absorption and antagonistic effects had been reported between the hydrocarbon carotenoids and oxycarotenoids; special carotenoids binding proteins had been isolated and purified from animal tissues, which could be helpful to explain the mechanisms of selective absorption and interaction

关 键 词：分析分布 类胡萝卜素 类胡萝卜素结合蛋白 进展 

分 类 号：Q584[生物学—生物化学]