高效液相色谱法同时测定五指山绿茶中5种儿茶素类成分  

Simultaneous Determination of Five Catechins in Green Tea using High Performance Liquid Chromatography

作　　者：孙晓莉[1] 刘帅华[1] 汪雷[1] 李汶罡[1] 赵春建[1] 祖元刚[1] 

机构地区：[1]东北林业大学森林植物生态学教育部重点实验室,哈尔滨150040

出　　处：《植物研究》2011年第3期377-380,共4页Bulletin of Botanical Research

基　　金：国家"十一五"科技支撑计划项目(2006BAD18B04)

摘　　要：建立了同时测定五指山绿茶中表儿茶素（EC）、儿茶素（C）、表没食子儿茶素没食子酸酯（EGCG）、表儿茶素没食子酸（ECG）和表没食子儿茶素（EGC）的高效液相色谱法。色谱条件为：HIQ SIL C18 V色谱柱（250 mm×4.6 mm,5μm）;乙腈-1.5%冰乙酸体系为流动相进行梯度洗脱;紫外检测器,检测波长280 nm;流速为1.0 mL.min-1;进样量10μL;柱温为35℃。在优化条件下,5种儿茶素类成分的浓度分别在15～300、10～200、10～200、20～400和10～200μg.mL-1与色谱峰面积之间的线性关系良好;平均回收率在96.20%～99.64%之间;RSD在1.12%～2.24%之间。该方法简便、快速、准确,可用于绿茶中儿茶素的质量控制。A high performance liquid chromatography（HPLC） method was developed for the simultaneous determination of epicatechin（EC）,catechin（C）,epigallocatechin gallate（EGCG）,epicatechin gallate（ECG） and epigallocatechin（EGC） in Wuzhishan green tea.The separation was performed on a HIQ SIL C18 V column（250mm×4.6mm,5μm） using gradient elution with acetonitrile-1.5% acetic acid as the mobile phase.The sample was detected by an UV detector at 280nm,flow rate was 1.0mL·min-1,the sample volume was 10μL and column temperature was 35℃.Under the optimized conditions,there was good linearity between the logarithm values and the peak areas of EC,C,EGCG,ECG and EGC in the ranges of 15-300,10-200,10-200,20-400 and 10-200μg·mL-1,respectively.Recoveries of the five components were between 96.20% and 99.64%,the RSDs were between 1.12% and 2.24%.This method is simple,rapid,accurate and suitable for the quality control of green tea.

关 键 词：绿茶 儿茶素 HPLC 

分 类 号：Q946.841[生物学—植物学]